Immunofluorescent analysis of permeabilized HeLa cells labeling RPA4 with ab169032 at 10µg/ml.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human Spleen tissue labeling RPA4 with ab169032 at 3µg/ml.
All lanes : Anti-RPA4 antibody (ab169032) at 1 µg/mlLane 1 : RPA4-transfected 293T cell line lysateLane 2 : Non-transfected 293T cell line lysateSecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilutiondeveloped using the ECL technique
Anti-RPA4 antibody (ab169032) at 1 µg/ml + Human spleen lysate at 50 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilutiondeveloped using the ECL technique