All lanes : Anti-PROX1 antibody - C-terminal (ab76696) at 1/50 dilutionLane 1 : 293 cell lysateLane 2 : PROX1 transfected 293 cell lysateLysates/proteins at 2 µg per lane.
Anti-PROX1 antibody - C-terminal (ab76696) at 1/50 dilution + A2058 cell lysate at 35 µg
Immunofluorescence of A2058 cells labelling PROX1 with ab76696. A2058 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab76696 (1:25, 1 h at 37℃). Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used as the secondary antibody (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃) and nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). PROX1 immunoreactivity is localised to the nucleus.
ab76696, at 1/50 dilution, staining PROX1 in human hepatocarcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and AEC staining.