![All lanes : Anti-PROX1 antibody [5G10] (ab33219) at 10 µg/mlLane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate (ab14660)Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (negative control)Lysates/proteins at 20 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/17252_PROX1-Primary-antibodies-ab33219-21.jpg)
All lanes : Anti-PROX1 antibody [5G10] (ab33219) at 10 µg/mlLane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate (ab14660)Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (negative control)Lysates/proteins at 20 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ab33219, at a 1/50 dilution, staining human PROX1 in pancreas tissue by Immunohistochemistry, Formalin fixed Paraffin embedded tissue.See Abreview
![Anti-PROX1 antibody [5G10] (ab33219) at 10 µg/ml + P3 Dentate gyrus whole tissue lysate at 50 µgdeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/17254_PROX1-Primary-antibodies-ab33219-13.jpg)
Anti-PROX1 antibody [5G10] (ab33219) at 10 µg/ml + P3 Dentate gyrus whole tissue lysate at 50 µgdeveloped using the ECL techniquePerformed under reducing conditions.
![Overlay histogram showing A549 cells stained with ab33219 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab33219, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/17255_PROX1-Primary-antibodies-ab33219-23.jpg)
Overlay histogram showing A549 cells stained with ab33219 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab33219, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.