Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling PPP5 with ab71150 at 1/200 (1µg/ml). Detection: DAB.
All lanes : Anti-PP5 antibody (ab71150) at 0.04 µg/mlLane 1 : Whole cell lysate from Hela cells at 50 µgLane 2 : Whole cell lysate from Hela cells at 15 µgLane 3 : Whole cell lysate from Hela cells at 5 µgLane 4 : Whole cell lysate from 293T cells at 50 µgLane 5 : Whole cell lysate from NIH3T3 cells at 50 µg
Immunoprecipitation/ Western Blot of PP5 Lane 1: ab71150 at 3µg/mg whole cell lysate. Lane 2: IgG control IP Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Subsequent WB detection was performed using 1 µg/ml ab71150. Chemiluminescence with an exposure time of 5 seconds.