
Paraffin-embedded human brain tissue stained for phospholipase C beta 3, using ab52199 (1/100). The right hand panel represents a negative control in which ab52199 has been pre-incubated with the immunizing (blocking) peptide.

All lanes : Anti-Phospholipase C beta 3 antibody (ab52199) at 1/500 dilutionLane 1 : Extracts from HeLa cells treated with PMA (125ng/ml, 30min).Lane 2 : Extracts from HeLa cells treated with PMA (125ng/ml, 30min). For this panel, ab52199 was pre-incubated with the immunizing (blocking) peptide.