Western blot analysis of E2F-2 expression in phorbol ester-induced A-431 nuclear extracts (A,B). Antibodies tested include E2F-2 (L-20): sc-632 (A) and E2F-2 (C-20): sc-633 (B).
ChIP analysis of E2F transcription factor binding to the c-Myc promoter in NBS cells. Antibodies tested include E2F-1 (C-20): sc-193 (A), E2F-2 (C-20): sc-633 (B), E2F-3 (C-18): sc-878, E2F-3 (N-20): sc-879 (C) and E2F-4 (C-20): sc-866 (D). Data kindly provided by Dr. J. H. J. Petrini and reproduced with permission from Master, et al. 2001. Mol. Cell. Biol. 21: 6006-6016.
ChIP analysis of E2F transcription factor binding to the oriP (I.) and c-Myc (II.) promoters in Raji cells as a function of the cell cycle. Antibodies tested include E2F-1 (C-20): sc-193 (A), E2F-2 (C-20): sc-633 (B), E2F-3 (C-18): sc-878, E2F-3 (N-20): sc-879 (C) and E2F-4 (C-20): sc-866 (D). Data kindly provided by Dr. J. H. J. Petrini and reproduced from Master, et al., 2001. Mol. Cell. Biol. 21: 6006-6016.
E2F-2 (C-20): sc-633. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.