Anti-Nesprin3 antibody
| Name | Anti-Nesprin3 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab74261 |
| Prices | $379.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ELISA ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human |
| Antigen | A synthesized peptide derived from an internal region of human Nesprin3 |
| Description | Rabbit Polyclonal |
| Gene | SYNE3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Nesprin3 antibody (ab74261) at 1/500 dilutionLane 1 : Extracts from Jurkat cellsLane 2 : Extracts from Jurkat cells plus 5µg immunizing peptideLysates/proteins at 5 µg per lane.
ICC/IF image of ab74261 stained HeLa cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74261, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.