Anti-Lactoferrin antibody (ab77548) at 0.05 µg/ml + Human Peripheral Blood Mononucleocyte lysate (in RIPA buffer) at 35 µgObserved band size : 85 kDa (why is the actual band size different from the predicted?)
ab77548 (2µg/ml) staining of paraffin embedded Human Blood Vessel shows strong staining of cytoplasm of neutrophils. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Sandwich-type ELISA with increasing amount of recombinant LTF captured by a rabbit antibody. ab77548 reporter concentration: 5 ug/ml
Anti-Lactoferrin antibody (ab77548) at 0.2 µg/ml + Human Lactoferrin full length protein (ab78526) at 0.01 µg/mlSecondaryRabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 8 minutes