Anti-KMT4 / Dot1L antibody
| Name | Anti-KMT4 / Dot1L antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab64077 |
| Prices | $384.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF IHC-P |
| Species Reactivities | Mouse, Rat, Human, Monkey |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1700 to the C-terminus of Human KMT4/ Dot1L |
| Description | Rabbit Polyclonal |
| Gene | DOT1L |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Lanes 1 - 2 : Anti-KMT4 / Dot1L antibody (ab64077) at 5 µg/ml (1% Milk Block)Lanes 3 - 4 : Anti-KMT4 / Dot1L antibody (ab64077) at 2 µg/ml (1% Milk Block)Lane 1 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 2 : Liver (Mouse) Tissue LysateLane 3 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 4 : Liver (Mouse) Tissue LysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab64077 stained MCF-7 cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64077, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% Methanol fixed (5 min) HeLa, Hek293. HepG2 cells at 5µg/ml.
Product References
H3K79 methylation directly precedes the histone-to-protamine transition in - H3K79 methylation directly precedes the histone-to-protamine transition in
Dottermusch-Heidel C, Klaus ES, Gonzalez NH, Bhushan S, Meinhardt A, Bergmann M, Renkawitz-Pohl R, Rathke C, Steger K. Andrology. 2014 Sep;2(5):655-65.
Dynamics of DOT1L localization and H3K79 methylation during meiotic prophase I in - Dynamics of DOT1L localization and H3K79 methylation during meiotic prophase I in
Ontoso D, Kauppi L, Keeney S, San-Segundo PA. Chromosoma. 2014 Mar;123(1-2):147-64.
Involvement of DOT1L in the remodeling of heterochromatin configuration during - Involvement of DOT1L in the remodeling of heterochromatin configuration during
Ooga M, Suzuki MG, Aoki F. Biol Reprod. 2013 Dec 19;89(6):145.
Genome-wide association and functional studies identify the DOT1L gene to be - Genome-wide association and functional studies identify the DOT1L gene to be
Castano Betancourt MC, Cailotto F, Kerkhof HJ, Cornelis FM, Doherty SA, Hart DJ, Hofman A, Luyten FP, Maciewicz RA, Mangino M, Metrustry S, Muir K, Peters MJ, Rivadeneira F, Wheeler M, Zhang W, Arden N, Spector TD, Uitterlinden AG, Doherty M, Lories RJ, Valdes AM, van Meurs JB. Proc Natl Acad Sci U S A. 2012 May 22;109(21):8218-23. doi: