Anti-GilZ / TilZ antibody
| Name | Anti-GilZ / TilZ antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab55015 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | IHC-P WB ICC/IF ICC/IF FC ChIPseq |
| Species Reactivities | Mouse, Human |
| Antigen | Recombinant fragment: MAQSKLDCRS PVGLDCCNCC LDLAHRSGLQ RGSSGENNNP GSPTVSNFRQ LQEKLVFENL NTDKLNSIMR QDSLEPVLRD PCYLINEGIC NRNIDQT, corresponding to amino acids 1-98 of Human GilZ/ TilZ Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | TSC22D3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HeLa cells stained with ab55015 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55015, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/24485_ab55015-4-ab55015FC.jpg)
Overlay histogram showing HeLa cells stained with ab55015 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55015, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
GilZ / TilZ antibody (ab55015) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human lymph node.
Western blot against tagged recombinant protein immunogen using ab55015 GilZ / TilZ antibody at 1ug/ml.
ab55015 at 10 ug/ml staining GilZ / TilZ in human Hela cells by Immunocytochemistry/ Immunofluorescence.
Product References
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