RNAi-directed mRNA Cleavage
HLA-DRα siRNA and shRNA Plasmids (h)
- Target species: human; for corresponding mouse product, see HLA-DRα siRNA and shRNA Plasmids (m)
- CRISPR/Cas9 KO Plasmids consists of HLA-DRα-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library
- For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double strand break (DSB) in the genomic DNA
- Target-specific CRISPR Plasmids for both gene knockout and activation are available. Please refer to the detailed product information in the tabs below
- Gene knockdown or activation can be assayed using HLA-DRα Antibody (G-7): sc-55593
- All products are provided as transfection-ready, purified plasmid DNA, except the Lentiviral Activation Particles which have been prepackaged as Lentiviral Particles for use with hard-to-transfect cells
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Ordering Information
siRNA
Primer
shRNA Plasmid
shRNA Lentiviral Particles
Recommended Control Antibody
SEE ALSO...
HLA-DR α Antibodies for analysis of cellular responses to HLA-DR α si/shRNA Gene Silencers
Description
- 10 µM, 50-100 transfections
- siRNA products generally consist of pools of three to five target-specific 19-25 nt siRNAs designed to knockdown gene expression
- for independent verification of HLA-DRα gene silencing results, individual siRNA duplex components are also available upon request
- suitable control antibody: HLA-DRα Antibody (G-7): sc-55593
- sqRT-PCR Primer also available: HLA-DRα (h)-PR: sc-37113-PR, 20 µl
- Get details about si/shRNA Gene Silencers
See Protocol Online.
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Description
- 20 µl of each primer set is provided at a concentration of 10 µM
- HLA-DRα Primer (h) includes A set (forward & reverse) and B set (forward & reverse) primers
- Primer sets are recommended for semi-quantitative nested reverse transcriptase PCR
See Protocol Online.
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Description
- 20 µg, up to 20 transfections
- shRNA Plasmids generally consist of a pool of three to five lentiviral vector plasmids each encoding target-specific 19-25 nt (plus hairpin) shRNAs designed to knockdown gene expression
- shRNA sequences correspond to HLA-DRα siRNA Gene Silencer sequences
- provided as transfection-ready purified plasmid DNA target-specific gene silencing
- after transfection, cells stably expressing shRNAs can be isolated by puromycin selection
- shRNA Plasmids are intended only for non-commercial use.
- suitable control antibody: HLA-DRα Antibody (G-7): sc-55593
- sqRT-PCR Primer also available: HLA-DRα (h)-PR: sc-37113-PR, 20 µl
See Protocol Online.
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Description
- 200 µl viral stock containing 1x 106 infectious units of virus (IFU) , sufficient for 10-20 transductions
- Lentiviral Particles are provided as transduction-ready viral particles for gene silencing
- Lentiviral Particles generally contain three to five expression constructs each encoding target-specific 19-25 nt (plus hairpin) shRNA designed to knockdown gene expression
- shRNA sequences correspond to HLA-DRα siRNA Gene Silencer sequences
- After transduction, stable cell lines expressing the shRNA may be isolated via selection with puromycin
- Biosafety - Lentiviral Particles are replication-incompetent and are designed to self-inactivate after transduction and integration of shRNA constructs into genomic DNA of target cells.
- Lentiviral Particles are intended for non-commercial use.
See Protocol Online.
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Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HLA-DRα siRNA (h) | sc-37113 | 10 µM | $293.00 | |||
HLA-DRα (h)-PR | sc-37113-PR | 10 µM, 20 µl | $33.00 | |||
HLA-DRα shRNA Plasmid (h) | sc-37113-SH | 20 µg | $592.00 | |||
HLA-DRα shRNA (h) Lentiviral Particles | sc-37113-V | 200 µl | $711.00 |