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Recombinant Human PSMB10/MECL1 His Protein

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SDS-Page: Recombinant Human PSMB10/MECL1 His Protein [NBP1-72454] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.25 mg/ml

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Recombinant Human PSMB10/MECL1 His Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 40-273 of Human PSMB10/MECL1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MTTIAGLVFQ DGVILGADTR ATNDSVVADK SCEKIHFIAP KIYCCGAGVA ADAEMTTRMV ASKMELHALS TGREPRVATV TRILRQTLFR YQGHVGASLI VGGVDLTGPQ LYGVHPHGSY SRLPFTALGS GQDAALAVLE DRFQPNMTLE AAQGLLVEAV TAGILGDLGS GGNVDACVIT KTGAKLLRTL SSPTEPVKRS GRYHFVPGTT AVLTQTVKPL TLELVEETVQ AMEVE

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
PSMB10
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
26.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 40% glycerol, 0.1 M NaCl
Preservative
No Preservative
Concentration
0.25 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human PSMB10/MECL1 His Protein

  • beta2i
  • FLJ00366
  • LMP10
  • LMP10Proteasome subunit beta-2i
  • Low molecular mass protein 10
  • Macropain subunit MECl-1
  • MECL1
  • MECl-1
  • MECL1EC 3.4.25.1
  • MGC1665
  • Multicatalytic endopeptidase complex subunit MECl-1
  • proteasome (prosome, macropain) subunit, beta type, 10
  • proteasome catalytic subunit 2i
  • Proteasome MECl-1
  • proteasome subunit beta 7i
  • proteasome subunit beta type-10
  • Proteasome subunit beta-2i
  • proteasome subunit MECL1
  • PSMB10

Background

PSMB10 is a member of the proteasome B-type family, also known as the T1B family, which is a 20S core beta subunit. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. Expression of the PSMB10 gene is induced by gamma interferon, and PSMB10 replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome. Recombinant human PSMB10 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol PSMB10