Recombinant Human UbcH5a/UBE2D1 Protein Summary
| Description |
A recombinant protein corresponding to Human UbcH5a/UBE2D1 |
Preparation
Method |
>95% SDS-PAGE |
| Protein/Peptide Type |
Recombinant Protein |
| Gene |
UBE2D1 |
| Purity |
>95%, by SDS-PAGE |
Applications/Dilutions
| Theoretical MW |
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles. |
| Buffer |
Supplied at a concentration of 1.0 mg/ml in 20 mM Tris-HCl, pH 7.5, containing 0.5 mM DTT.
|
| Concentration |
1 mg/ml |
| Purity |
>95%, by SDS-PAGE |
Notes
The purity of this protein is >95% pure by SDS-PAGE
Alternate Names for Recombinant Human UbcH5a/UBE2D1 Protein
Background
UbcH5a is highly homologous to the S. cerevisiae Ubc4/5 family of E2 enzymes demonstrated to be essential for degradation of regulatory and abnormal proteins. UbcH5a, together with UbcH5b and UbcH5c, are the most active class of E2 enzymes in cell extracts and are associated with regulation of a number of transcription factors. Specifically, UbcH5a stimulates the conjugation of ubiquitin to the tumor suppressor p532. Useful for in vitro ubiquitinylation reactions. Typical enzyme concentration to support conjugation in vitro is 100 nM to 1uM depending upon conditions.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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