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Recombinant Human Caspase-8 Protein

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Summary
Product Discontinued
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    • Catalog Number
      NBP1-99587
    • Availability
      Product Discontinued

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Recombinant Human Caspase-8 Protein Summary

Description
A recombinant protein corresponding to Human Caspase-8
Preparation
Method
>95% SDS-PAGE
Details of Functionality
Specifications/Test method: > 5000 units/mg
Protein/Peptide Type
Recombinant Protein
Gene
CASP8
Purity
>95%, by SDS-PAGE

Applications/Dilutions

Application Notes
Unit Definition: One unit of the recombinant caspase-8 is the enzyme activity that cleaves 1 nmol of the caspase substrate IETD-pNA (pNA: pnitroanaline) per hour at 37C in a reaction solution containing 50 mM Hepes pH 7.2 50 mM NaCl 0.1% Chaps 10 mM EDTA 5% Glycerol and 10 mM DTT.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Concentration
Lyoph
Purity
>95%, by SDS-PAGE
Reconstitution Instructions
Reconstitute with PBS containing 15% glycerol to a final concentration of 1 unit per ul.

Notes

The purity of this protein is >95% pure by SDS-PAGE

Alternate Names for Recombinant Human Caspase-8 Protein

  • AIS
  • androgen receptor
  • CASP8
  • Caspase8
  • Caspase-8
  • DHTRTFM
  • Dihydrotestosterone receptorHYSP1
  • HUMARA
  • Mch5
  • NR3C4KD
  • Nuclear receptor subfamily 3 group C member 4
  • SMAX1SBMA
  • spinal and bulbar muscular atrophy

Background

Caspase-8 (also know as FLICE, MASH, Mch5) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-8 also exists in cells as an inactive proenzyme. During apoptosis procaspase-8 is processed at aspartate residues by self-proteolysis and/or cleavage by another caspase. The processed active form of caspase-8 consists of large and small subunits which associate to form the active enzyme. Active caspase-8 has been shown to activate caspase-3 leading to degradation of a variety of cellular target proteins during apoptosis. The recombinant active human caspase-8 was expressed in E. coli. The active caspase-8 is routinely tested at for its ability to enzymatically cleave these two substrates Ac-IETD-pNA (Cat. #1063-200) or Ac-IETD-AFC (1062-200).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Contact Information

Novus USA
Phone: 1-888-506-6887
Phone: 303-730-1950
Fax: 303-730-1966
Email: novus@novusbio.com
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Bioinformatics

Gene Symbol CASP8