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Recombinant Human IL-12 Protein

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Summary
Product Discontinued
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    • Catalog Number
      NBP1-99345
    • Availability
      Product Discontinued

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Recombinant Human IL-12 Protein Summary

Description
Recombinant bioactive protein for Human IL-12

Source:CHO cells
Amino Acid Sequence:(P29459)
Preparation
Method
Determined to be >98% pure by SDS-PAGE
Details of Functionality
The ED50 as determined by the induction of IFN-g production from murine splenocytes co-stimulated with IL-12 is <0.1 ng/ml, corresponding to a specific activity of > 1 x 107 units/mg.
Source
CHO
Protein/Peptide Type
Recombinant Protein
Gene
IL12A
Purity
>98%, by SDS-PAGE
Endotoxin Note
<0.1 ng/ug

Applications/Dilutions

Dilutions
  • Bioactivity
  • SDS-Page
Theoretical MW
75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C.
Buffer
Lyophilized from 0.5X PBS (pH 7.2)
Concentration
Lyoph
Purity
>98%, by SDS-PAGE
Reconstitution Instructions
Reconstitute in 1X PBS (pH 7.2-7.4) to a concentration of 0.2 mg/ml. The solution can then diluted into other aqueous buffers andstored at 4C for 1 week or -20C for long-term storage.

Alternate Names for Recombinant Human IL-12 Protein

  • CLMF p35
  • CLMF1
  • Cytotoxic lymphocyte maturation factor 35 kDa subunit
  • IL-12 subunit p35
  • IL12
  • IL-12
  • IL-12, subunit p35
  • IL12A
  • interleukin 12, p35
  • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35)
  • interleukin-12 alpha chain
  • interleukin-12 subunit alpha
  • natural killer cell stimulatory factor 1, 35 kD subunit
  • NF cell stimulatory factor chain 1
  • NK cell stimulatory factor chain 1
  • NKSF1
  • p35

Background

IL-12 is a potent regulator of cell mediated immune responses and in induces IFN-g production by NK and T cells. It is produced by activated monocytes/macrophage cells, B-lymphocytes, and connective tissue type mast cells. Among its biological activities, IL-12 promotes the growth and activity of activated NK, CD4+, and CD8+ cells and induce the development of IFN-g producing Th1 cells. Recombinant human IL-12 is a 75 kDa heterodimeric glycoprotein consisting of disulfide-linked 35 kDa (p35) and 40 kDa (p40) subunits (503 total amino acid residues).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol IL12A