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Recombinant Rat EGF Protein

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Summary
Product Discontinued
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    • Catalog Number
      NBP1-99896
    • Availability
      Product Discontinued

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Recombinant Rat EGF Protein Summary

Description
A recombinant protein corresponding to 53 amino acids of Rat EGF

Amino Acid Sequence: NSNTGCPPSYDGYCLNGGVCMYVESVDRYVCNCVIGYIGERCQHRDLRWWKLR
Preparation
Method
>98% SDS-PAGE
Details of Functionality
Biological Activity: The ED50 as calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is less than 0.1ng/ml, corresponding to a specific activity of > 1 x 10^7units/mg.
Results: <0.1 ng/ml
Protein/Peptide Type
Recombinant Protein
Gene
EGF
Purity
>98%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
6.15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
From PBS, pH 7.4.
Concentration
Lyoph
Purity
>98%, by SDS-PAGE
Reconstitution Instructions
Centrifuge prior to opening. Reconstitute with sterilized water to a concentration greater than or equal to 0.1 mg/ml. This solution can then be diluted into other aqueous buffer.

Notes

The purity of this protein is >95% pure by SDS-PAGE

Alternate Names for Recombinant Rat EGF Protein

  • beta-urogastrone
  • EGF
  • epidermal growth factor (beta-urogastrone)
  • epidermal growth factor
  • hEGF
  • HOMG4
  • pro-epidermal growth factor
  • URG
  • Urogastrone

Background

Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Epidermal Growth Factor Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 53 amino acids and having a molecular mass of 6151 Dalton. The Rat EGF is purified by proprietary chromatographic techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol EGF