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Recombinant Mouse CD40 Ligand/TNFSF5 Protein

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Summary
Product Discontinued
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    • Catalog Number
      NBP1-99265
    • Availability
      Product Discontinued

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Recombinant Mouse CD40 Ligand/TNFSF5 Protein Summary

Description
A recombinant protein corresponding to 149 amino acids of Mouse CD40 Ligand/TNFSF5
Preparation
Method
>98% SDS-PAGE
Details of Functionality
Biological Activity: ED50 is <0.1 ug/ml determined by its ability to induce TNF-a and MIP-1a production by murine splenocytes.
Results: <0.1 ug/ml
Protein/Peptide Type
Recombinant Protein
Gene
CD40LG
Purity
>98%, by SDS-PAGE
Endotoxin Note
<0.1 ng/ug

Applications/Dilutions

Theoretical MW
16.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Sterile filtered and then10 mM Sodium Phosphate, pH 7.5 + 0.1 M Arginine.
Concentration
Lyoph
Purity
>98%, by SDS-PAGE
Reconstitution Instructions
Reconstitute with water to a final concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers.

Notes

The purity of this protein is >95% pure by SDS-PAGE

Alternate Names for Recombinant Mouse CD40 Ligand/TNFSF5 Protein

  • CD154 antigen
  • CD154
  • CD40 antigen ligand
  • CD40 Ligand
  • CD40L
  • CD40-L
  • CD40LG
  • CD40LIGM
  • gp39
  • hCD40L
  • HIGM1
  • T-B cell-activating molecule
  • T-BAM
  • T-cell antigen Gp39
  • TNF-related activation protein
  • TNFSF5
  • TNFSF5IMD3
  • TRAP
  • TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome)
  • tumor necrosis factor (ligand) superfamily member 5
  • Tumor necrosis factor ligand superfamily member 5

Background

CD40 Ligand (CD40L/TRAP) is a type II transmembrane glycoprotein belonging to the TNF superfamily of ligands. Natually, CD40L is a membrane-bound protein, but proteolytically cleaved 15-18 kDa soluble forms of CD40L are also fully active. CD40L plays an important role in the proliferation and differentiation of B-cells, and is critical for immunoglobulin (Ig) class switching. Recombinant murine CD40 Ligand is a soluble 18 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CDE40L.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol CD40LG