Recombinant Human Wnt-1 Protein

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Summary
Product Discontinued
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    • Catalog Number
      P4134
    • Availability
      Product Discontinued

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Recombinant Human Wnt-1 Protein Summary

Description
Partial recombinant protein corresponding to343 amino acids of Human WNT1

Source: Escherichia coli

Amino Acid Sequence:P04628

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
WNT1
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Note
The ED50 was determined by its ability to enhance BMP-2 induced alkaline phosphatase production by murine ATDC5 cells. The expected ED50 for this effect is 1.5-2.5 ng/mL in the presence of 200 ng/mL of human BMP-2.

Applications/Dilutions

Dilutions
  • Bioactivity
  • Functional
  • SDS-Page
Theoretical MW
38.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
No additive
Preservative
No Preservative
Concentration
LYOPH
Purity
>95%, by SDS-PAGE and HPLC
Reconstitution Instructions
Reconstitute with distilled water.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human Wnt-1 Protein

  • Int-1
  • INT1Proto-oncogene Int-1 homolog
  • proto-oncogene Wnt-1
  • wingless-type MMTV integration site family, member 1 (oncogene INT1)
  • wingless-type MMTV integration site family, member 1
  • Wnt1
  • Wnt-1

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is very conserved in evolution, and the protein encoded by this gene is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. This gene is clustered with another family member, WNT10B, in the chromosome 12q13 region. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol WNT1