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Recombinant Human Apolipoprotein A-I/ApoA1 Protein

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SDS-Page: Recombinant Human Apolipoprotein A-I/ApoA1 Protein [NBP2-34869]
SDS-Page: Recombinant Human Apolipoprotein A-I/ApoA1 Protein [NBP2-34869]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Format
Carrier-Free
Concentration
LYOPH

Order Details

  • Size / Catalog#QtyPrice
    NBP2-34869-100ug
    100 ug
    $333.00
    10-12 Business Days
    NBP2-34869-500ug
    500ug
    $699.00
    10-12 Business Days
    NBP2-34869-1mg
    1mg
    $1,200.00
    10-12 Business Days
    View Available Formulations
    Catalog# & Formulation Size Price
    Carrier-Free
    NBP2-34869-1mg
    Carrier-Free
    NBP2-34869-500ug
Shipping $45.00 per order
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Recombinant Human Apolipoprotein A-I/ApoA1 Protein Summary

Description
A single non-glycosylated polypeptide chain containing 243 amino acids corresponding to Apolipoprotein A-I/ApoA1 Source: E. coli

Uniprot ID: P02647

Amino Acid Sequence: DEPPQSPWDR VKDLATVYVD VLKDSGRDYV SQFEGSALGK QLNLKLLDNW DSVTSTFSKL REQLGPVTQE FWDNLEKETE GLRQEMSKDL EEVKAKVQPY LDDFQKKWQE EMELYRQKVE PLRAELQEGA RQKLHELQEK LSPLGEEMRD RARAHVDALR THLAPYSDEL RQRLAARLEA LKENGGARLA EYHAKATEHL STLSEKAKPA LEDLRQGLLP VLESFKVSFL SALEEYTKKL NTQ
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
APOA1
Purity
> 98 % pure by SDS-PAGE and HPLC
Endotoxin Note
Less than 0.1 EU/ug of Apolipoprotein A-I/ApoA1 as determined by LAL method.

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
28.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative
No Preservative
Concentration
LYOPH
Purity
> 98 % pure by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human Apolipoprotein A-I/ApoA1 Protein

  • Alp-1
  • APOA1
  • Apo-AI
  • apoA-I
  • Apolipoprotein A1
  • Apolipoprotein AI
  • Apolipoprotein A-I
  • Brp-14
  • Ltw-1
  • Lvtw-1
  • MGC117399
  • Sep-1
  • Sep-2

Background

Apolipoprotein A-I encoded by the APOA1 gene is the major protein component of high density lipoprotein (HDL) in plasma. It has a specific role in lipid metabolism. Apolipoprotein A-I participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). Apolipoprotein A-I is also isolated as a prostacyclin (PGI2) stabilizing factor and thus may have an anticlotting effect. Apolipoprotein A-I has been shown to interact with ABCA1, GPLD1 and PLTP. Apolipoprotein A-I binds to lipopolysaccharide or endotoxin and has a major role in the anti-endotoxin function of HDL. Mature human ApoA-1 shares 65 % and 62 % a.a. sequence identity with murine and rat ApoA-1, respectively. It has an approximate molecular weight of 28.1 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol APOA1
Uniprot