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Recombinant Mouse IL-1 alpha/IL-1F1 Protein

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SDS-Page: Mouse IL-1 alpha/IL-1F1 Protein [NBP2-35107]
SDS-Page: Mouse IL-1 alpha/IL-1F1 Protein [NBP2-35107]

Product Details

Summary
Reactivity MuSpecies Glossary
Applications PAGE, Bioactivity
Format
Carrier-Free
Concentration
LYOPH

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Catalog# & Formulation Size Price
Carrier-Free
NBP2-35107-10ug
Carrier-Free
NBP2-35107-1mg
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NBP2-35107-500ug
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Recombinant Mouse IL-1 alpha/IL-1F1 Protein Summary

Description
A single non-glycosylated polypeptide chain containing 156 amino acids corresponding to IL-1 alpha/IL-1F1 Source: E. coli

Uniprot ID: Q3U0Y6

Amino Acid Sequence: SAPYTYQSDL RYKLMKLVRQ KFVMNDSLNQ TIYQDVDKHY LSTTWLNDLQ QEVKFDMYAY SSGGDDSKYP VTLKISDSQL FVSAQGEDQP VLLKELPETP KLITGSETDL IFFWKSINSK NYFTSAAYPE LFIATKEQSR VHLARGLPSM TDFQIS
Details of Functionality
IL1 alpha Protein is Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 2 pg/ml, corresponding to a specific activity of > 5.0 x 10^8 IU/mg.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
IL1A
Purity
> 97 % pure by SDS-PAGE and HPLC
Endotoxin Note
Less than 1 EU/ug of IL-1 alpha/IL-1F1 as determined by LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • SDS-Page
Theoretical MW
17.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-35107.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered solution in PBS (pH 7.4)
Preservative
No Preservative
Concentration
LYOPH
Purity
> 97 % pure by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Mouse IL-1 alpha/IL-1F1 Protein

  • BAF
  • Hematopoietin-1
  • IL1 alpha
  • IL-1 alpha
  • IL1
  • IL1A
  • IL-1A
  • IL1-ALPHA
  • IL1F1
  • IL-1F1
  • IL1F1hematopoietin-1
  • interleukin 1, alpha
  • interleukin-1 alpha
  • LAF
  • LEM
  • preinterleukin 1 alpha
  • pro-interleukin-1-alpha

Background

Interleukin-1 alpha (IL-1alpha) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1alpha and IL-1beta binds to the same receptor and has similar if not identical biological properties. Among various species, the amino acid sequence of mature IL-1alpha s conserved 60 % to 70 % and human IL1 has been found to be biologically active on murine cell lines .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol IL1A
Uniprot