Recombinant Human His10-cIAP-1/HIAP-2 Protein, CF

Name: Recombinant Human His10-cIAP-1/HIAP-2 Protein, CF
Brand: R&D Systems
SKU: E3-280
Price: 211 USD

Catalog #: E3-280 Datasheet / COA / SDS

Discontinued Product

E3-280 has been discontinued.

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Recombinant Human His10-cIAP-1/HIAP-2 Protein, CF Summary

Product Specifications

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Activity

Recombinant Human cIAP-1/HIAP-2 is a RING finger Ubiquitin ligase (E3) that functions downstream of a Ubiquitinactivating (E1) enzyme and a Ubiquitin-conjugating (E2) enzyme to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial cIAP-1 concentration of 0.2-1 μM.

Source

E. coli-derived human cIAP-1/HIAP-2 protein
Contains an N-terminal 10-His tag

Accession #

Q13490

Predicted Molecular Mass

72 kDa

Product Datasheets

E3-280

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E3-280

Formulation	X mg/ml (X μM) in 50 mM HEPES pH 8.0, 500 mM NaCl, 20% Glycerol (v/v), 5 mM TCEP, 0.5 mM EDTA
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: cIAP-1/HIAP-2

Cellular inhibitor of apoptosis protein 1 (cIAP-1, also known as BIRC2, MIHB, and HIAP2) is a member of the inhibitor of apoptosis (IAP) family of proteins that inhibit the proteolytic activity of mature caspases. Structurally, cIAP-1 is comprised of 3 BIR (baculovirus inhibitor of apoptosis) domains, a RING finger domain, and a caspase recruitment domain (CARD). Functionally, cIAP-1 inhibit caspases through the direct interaction of its BIR domain with the active caspase. The ring finger domain of cIAP-1 also functions as an E3 ubiquitin ligase to ubiquitinate specific target proteins. Caspase activity may be restored by mitochondrial proteins, such as SMAC/Diablo or HtrA2/Omi, through interactions with the Reaper-like motif and the BIR domain.

References

Gyrd-Hansen M. & Meier P. (2010) Nat. Rev. Cancer 10: 561
Bertrand M.J, et al. (2011) PLoS ONE 6: E22356
Kulathila R., et al. (2009) Acta Crystallogr. D 65: 58
Lopez J., et al. (2011) Mol. Cell 42: 569

Long Name

Cellular Inhibitor of Apoptosis Protein 1

Entrez Gene IDs

329 (Human); 11797 (Mouse)

Alternate Names

API1Hiap-2; apoptosis inhibitor 1; baculoviral IAP repeat containing 2; baculoviral IAP repeat-containing 2; baculoviral IAP repeat-containing protein 2; BIRC2; cIAP1; c-IAP1; cIAP-1; hIAP2; HIAP-2; IAP homolog B; IAP2; IAP-2; Inhibitor of apoptosis protein 2; MIHB; MIHBHIAP2; NFR2-TRAF signalling complex protein; RING finger protein 48; RNF48hiap-2; TNFR2-TRAF-signaling complex protein 2