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Recombinant Human Pro-GABARAPL1 Protein, CF

Catalog #: UL-400 Datasheet / COA / SDS

Discontinued Product

UL-400 has been discontinued.
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Recombinant Human Pro-GABARAPL1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity

pro-GABARAPL1 is an immature form of the protein that includes C-terminal Lys 117. This form is not competent for in vitro conjugation reactions as supplied. This protein may be used as a negative control for conjugation reactions, or as a substrate for the processing enzyme ATG4B.

Source
E. coli-derived human GABARAPL1 protein
Accession #
Q9H0R8
Predicted Molecular Mass
14 kDa

Product Datasheets

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UL-400

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

UL-400

Formulation

X mg/ml (X μM) in 50 mM HEPES, pH 7.5
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Background: GABARAPL1

Pro-gamma -Aminobutyric Acid Receptor-associated Protein-like 1 (GABARAPL1) is a 117 amino acid (aa) protein with a predicted molecular weight of 14 kDa. It is a member of the GABARAP subfamily of the Autophagy-related 8 (Atg8) family of proteins. GABARAPL1 has 100% aa sequence identity with its mouse and rat orthologs and is orthologous to yeast Atg8. Atg8 family members show structural similarity with Ubiquitin, but lack aa sequence similarity. GABARAPL1 was first described as the human ortholog of the guinea pig gec1 gene product, and is now known for its role in autophagy.  Mature GABARAPL1 (in which Lys 117 has been cleaved off by the ATG4B processing enzyme) covalently attaches to phosphatidylethanolamine (PE) in the phagophore (autophagosome precursor) membrane using a Ubiquitin-like conjugation system that includes Ubiquitin-activating (E1)-, Ubiquitin-conjugating (E2)-, and Ubiquitin Ligase (E3)-like enzymes. GABARAPL1 is involved in the late stages of autophagosome maturation, and may also be involved in cargo recruitment to autophagosomes.

References
  1. Le Grand, J.N. et al. (2011) Autophagy 7:1098
  2. Shpilka, T. et al. (2011) Genome Biol. 12:226.
  3. Vernier-Magnin, S. et al. (2001) Biochem. Biophy. Res. Com. 284:118.
  4. Weidberg, H. et al. (2010) EMBO J. 29:1792.
  5. Weidberg, H. et al. (2011) Ann. Rev. Biochem. 80:125.
Long Name
gamma-Aminobutyric Acid Type A Receptor Associated Protein Like 1
Entrez Gene IDs
23710 (Human); 57436 (Mouse); 689161 (Rat)
Alternate Names
APG8L; APG8-LIKE; ATG8; ATG8B; ATG8L; Early estrogen-regulated protein; GABA(A) receptor-associated protein like 1; GABARAPL1; gamma-aminobutyric acid receptor-associated protein-like 1; GEC1; GEC-1; GEC1GABA(A) receptor-associated protein-like 1; Glandular epithelial cell protein 1

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