Recombinant Mouse Ephrin-B1 Fc Chimera Biotinylated Protein

Catalog #: BT473
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
BT473	In Stock	25 ug / $385

Bulk Orders

R&D Systems Recombinant Proteins and Enzymes

1 Image

Product Details

Citations (1)

Supplemental Products

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Ephrin-B1 Fc Chimera Biotinylated Protein Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Activity

Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse EphB3 Fc Chimera at 2 µg/mL (100 µL/well) can bind biotinylated Recombinant Mouse Ephrin‑B1 Fc Chimera with a linear range of 0.078-1.25 ng/mL.
Optimal dilutions should be determined by each laboratory for each application.

Source

Mouse myeloma cell line, NS0-derived mouse Ephrin-B1 protein

Human Ephrin-B1 (Lys30-Ser229) Accession # Q544L9	IEGRMD	Human IgG₁ (Pro100-Lys330)	6-His tag
N-terminus			C-terminus

Accession #

Q544L9

N-terminal Sequence
Analysis

Lys30

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

49.2 kDa (monomer)

SDS-PAGE

60 kDa, reducing conditions

Product Datasheets

BT473

Product Datasheet

COA

SDS

BT473

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute with sterile PBS at 100 μg/mL.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Ephrin-B1

Ephrin-B1, also known as Elk Ligand, LERK2, and Eplg2, is an approximately 45 kDa member of the Ephrin-B family of transmembrane ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. The extracellular domains (ECD) of Ephrin-B ligands are structurally related to GPI-anchored Ephrin-A ligands. Eph‑Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. Ephrin-B1 preferentially interacts with receptors in the EphB family. The binding of Ephrin-B1 to EphB proteins also triggers reverse signaling through Ephrin-B1 (1, 2). Mature mouse Ephrin-B1 consists of a 212 amino acid (aa) ECD, a 21 aa transmembrane segment, and an 88 aa cytoplasmic domain (3, 4). Within the ECD, mouse Ephrin-B1 shares 94% and 98% aa sequence identity with human and rat Ephrin-B1, respectively. Ligation by EphB2 enhances shedding of a 35 kDa fragment of the Ephrin-B1 ECD (5). The residual membrane-bound portion is then cleaved by gamma-secretase to release the intracellular domain (6). Ephrin-B1 also associates in cis with Claudin-1, -4, and -5 (7, 8). It is expressed on glomerular podocyte slit diaphragms, developing thymocytes, peripheral T cells, monocytes, macrophages, vascular endothelial cells, cardiomyocytes, osteoclasts, and luteinizing granulosa cells in the ovary (8-13). In the developing nervous system, Ephrin-B1 plays a role in cellular migration, axon guidance, and presynaptic development (14-16). It also regulates developing thymocyte survival, monocyte migration, osteoclast differentiation and function, cardiac muscle morphogenesis, and tumorigenesis (5, 8, 10-12). Ephrin-B1 is up‑regulated on reactive astrocytes and on macrophages and T cells found in atherosclerotic plaques (11, 17).

References

Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
Shao, H. et al. (1994) J. Biol. Chem. 269:26606.
Fletcher, F.A. et al. (1994) Genomics 24:127.
Tanaka, M. et al. (2007) J. Cell Sci. 120:2179.
Tomita, T. et al. (2006) Mol. Neurodegen. 1:2.
Tanaka, M. et al. (2005) EMBO J. 24:3700.
Genet, G. et al. (2012) Circ. Res. 110:688.
Hashimoto, T. et al. (2007) Kidney Int. 72:954.
Yu, G. et al. (2006) J. Biol. Chem. 281:10222.
Sakamoto, A. et al. (2008) Clin. Sci. 114:643.
Cheng, S. et al. (2012) PLoS ONE 7:e32887.
Egawa, M. et al. (2003) J. Clin. Endocrinol. Metab. 88:4384.
Davy, A. et al. (2004) Genes Dev. 18:572.
Bush, J.O. and P. Soriano (2009) Genes Dev. 23:1586.
McClelland, A.C. et al. (2009) Proc. Natl. Acad. Sci. USA 106:20487.
Wang, Y. et al. (2005) Eur. J. Neurosci. 21:2336.

Entrez Gene IDs

1947 (Human); 13641 (Mouse); 25186 (Rat)

Alternate Names

Cek5-L; EFL3; EFL-3; EFNB1; ELK ligand; ELK-L; EphrinB1; Ephrin-B1; LERK-2; STRA-1

Related Research Areas

Citation for Recombinant Mouse Ephrin-B1 Fc Chimera Biotinylated Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

EphrinB1 is essential in T-cell-T-cell co-operation during T-cell activation.
Authors: Yu G, Luo H, Wu Y, Wu J
J. Biol. Chem., 2004-10-22;279(53):55531-9.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry