PSRC2 Recombinant Protein Antigen

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PSRC2 Recombinant Protein Antigen Summary

Description
A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human ZFC3H1.

Source: E. coli

Amino Acid Sequence: PSRKQNYSSKNENCVEETFEDLLLKYKQIQLELECINKDEKLALSSKEENVQEDPKTLNFEDQTSTDNVSITKDSSKEVAPEEKTQVKTFQAFELKPLRQKLTLPGDKNRLKKVKDGAKPLSL

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

Source
E. coli
Protein/Peptide Type
Recombinant Protein Antigen
Gene
ZFC3H1
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Dilutions
  • Antibody Competition 10 - 100 molar excess
Application Notes
This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP1-88166.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

Theoretical MW
32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 1M Urea, pH 7.4.
Preservative
No Preservative
Purity
>80% by SDS-PAGE and Coomassie blue staining

Alternate Names for PSRC2 Recombinant Protein Antigen

  • coiled-coil domain containing 131
  • Coiled-coil domain-containing protein 131
  • DKFZp686A0722
  • KIAA0546CCDC131
  • MGC23401
  • MGC90200
  • proline/serine-rich coiled-coil 2
  • Proline/serine-rich coiled-coil protein 2
  • PSRC2
  • zinc finger C3H1 domain-containing protein
  • zinc finger, C3H1-type containing

Background

CCDC131 (coiled-coil domain containing protein 131) is an uncharacterized proline/serine rich coiled-coil protein that bears 6 HAT repeats- and 5 TPR repeats. Coiled-coil proteins exhibit tertiary structures made up of a number of highly organized alpha helices wound around each other to mediate oligomerization. HAT (half a TPR) repeats form amphipathic alpha-helices and are similar to TPR repeats which are 34 amino acid repeat motifs which form alpha helical structures.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol ZFC3H1