CYP1B1 (h): 293T Lysate: sc-158414

CYP1B1 (h): 293T Lysate is derived from the 293T cell line, a human embryonic kidney cell line modified to express the SV40 large T antigen, which enhances its transfection efficiency and protein production capabilities. This lysate is specifically enriched with human Cytochrome P450 1B1 (CYP1B1), an enzyme that plays a crucial role in the metabolism of various xenobiotics and endogenous substrates, including the oxidative metabolism of polycyclic aromatic hydrocarbons (PAHs), steroids, and retinoids. CYP1B1 is involved in the bioactivation of procarcinogens and the detoxification of potentially harmful compounds, contributing to cellular defense mechanisms against environmental toxins. In research, CYP1B1 (h): 293T Lysate is utilized to study the enzyme's metabolic pathways and regulatory mechanisms. Researchers employ this lysate in Western blotting to detect and quantify CYP1B1 expression under different experimental conditions, providing insights into its induction by environmental contaminants and regulatory factors. The lysate is also used in enzymatic activity assays to measure the metabolism of specific substrates, helping to explain the catalytic properties and substrate specificity of CYP1B1. Additionally, CYP1B1 (h): 293T Lysate is valuable in co-immunoprecipitation assays to identify protein-protein interactions involving CYP1B1, which aids in understanding its integration within larger metabolic and signaling networks. Functional assays using this lysate can assess the impact of various inducers and inhibitors on CYP1B1 activity, contributing to a deeper understanding of its role in chemical metabolism and detoxification processes.