Home » STK35 » STK35 Lysates » STK35 Overexpression Lysate

STK35 Overexpression Lysate

 Submit a Review

Images

 
•
Image 1 of 1
(
Enlarge)
Western Blot: STK35 Overexpression Lysate (Adult Normal) [NBP2-10235] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for STK35.
Western Blot: STK35 Overexpression Lysate (Adult Normal) [NBP2-10235] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for STK35.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

  • Loading...
Shipping $45.00 per order
Novus Biologicals offers 100% Guarantee
Datasheet
Reviews & Publications
Protocols & FAQs
Support & Research

STK35 Overexpression Lysate Summary

Description

STK35 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC229271

Accession#: NM_080836

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
STK35

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
57.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for STK35 Overexpression Lysate

  • bA550O8.2
  • CLIK-1
  • CLIK1Serine/threonine-protein kinase 35 L1
  • CLP-36 interacting kinase
  • CLP-36-interacting kinase 1
  • EC 2.7.11
  • EC 2.7.11.1
  • PDIK1
  • PDLIM1-interacting kinase 1
  • serine threonine kinase 35 long form
  • serine/threonine kinase 35
  • serine/threonine-protein kinase 35
  • STK35L1

Background

STK35 associates with the PDZ-LIM protein CLP36 through the LIM domain and undergoes autophosphorylation in vitro. STK35 localizes predominantly to the nucleus; however, cotransfection of STK35 with CLP36 demonstrated redistribution of STK35 to the cytoplasm and into actin stress fibers, where it colocalizes with CLP36. The presence of STK35 disrupted the periodic staining pattern of CLP36 in these fibers. It is suggested that CLP36 specifically targets STK35 to actin stress fibers and that STK35 may represent a regulator of the actomyosin cytoskeleton in nonmuscle cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Recombinant Human
PDIK1L GST (N-Term) P ...
H00149420-P01
SDS-PAGE Recombinant Human PDIK1L GST (N-Term) Protein
Species: Hu
Applications: ELISA, AP, PA, PAGE, WB
p19ARF/CDKN2A
Antibody - BSA Free
NB200-106
Immunohistochemistry p19ARF/CDKN2A Antibody - BSA Free
•••
(1 Review)
(48 Publications)
Species: Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB

Customers Who Bought This Also Bought

Immunohistochemistry: p19ARF/CDKN2A Antibody [NB200-106] - Adult mouse kidney (glomerulus). Image from verified customer review.
p19ARF/CDKN2A Antibody - BSA Free
NB200-106

Contact Information


Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our STK35 Overexpression Lysate and receive a gift card or discount.


Bioinformatics

Gene Symbol STK35