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Human SNX1 ELISA Kit

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  • Alternative name

    Human HsT17379 ELISA Kit;Human VPS5 ELISA Kit;Human sorting nexin 1 ELISA Kit;Human sorting nexin-1 ELISA Kit;Human sorting nexin 1A ELISA Kit;
  • Catalog
    E036198
  • species
    Human
  • GeneSNX1
  • Standard CurveHuman SNX1 ELISA Kit
  • Other Species Mouse Snx1 ELISA KitBovine SNX1 ELISA KitMonkey SNX1 ELISA KitRat Snx1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of SNX1. No significant cross-reactivity or interference between SNX1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SNX1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman SNX1 ELISA Kit allows for the in vitro quantitative determination of SNX1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: SNX1 ELISA kit applies the competitive enzyme immunoassay technique utilizing SNX1 antigen and an SNX1-Ab-HRP conjugate. The assay sample and buffer are incubated together with SNX1-Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SNX1 concentration since SNX1 from samples and SNX1 pre-coated on the plate compete for the SNX1 Ab-HRP binding site.Since the number of sites is limited, as more sites are occupied by SNX1 from the sample, fewer sites are left to bind SNX1 pre-coated on the plate.A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SNX1 concentration in each sample is interpolated from this standard curve.
  • Human Sorting nexin-1 Protein information
  • Uniprot ID SNX1_HUMAN
  • Uniprot AC Q13596; A6NM19; A8K6T7; H0Y2M5; O60750; O60751; Q6ZRJ8;
  • UniGene Hs.188634;
  • GeneID 6642
  • KEGG hsa:6642;
  • Human Sorting nexin-1 Protein SEQUENCE

  • SEQUENCE 522 AA; 59070 MW; 6AC8AA0A589513E3 CRC64;

    MASGGGGCSA SERLPPPFPG LEPESEGAAG GSEPEAGDSD TEGEDIFTGA

    AVVSKHQSPK ITTSLLPINN GSKENGIHEE QDQEPQDLFA DATVELSLDS

    TQNNQKKVLA KTLISLPPQE ATNSSKPQPT YEELEEEEQE DQFDLTVGIT

    DPEKIGDGMN AYVAYKVTTQ TSLPLFRSKQ FAVKRRFSDF LGLYEKLSEK

    HSQNGFIVPP PPEKSLIGMT KVKVGKEDSS SAEFLEKRRA ALERYLQRIV

    NHPTMLQDPD VREFLEKEEL PRAVGTQTLS GAGLLKMFNK ATDAVSKMTI

    KMNESDIWFE EKLQEVECEE QRLRKLHAVV ETLVNHRKEL ALNTAQFAKS

    LAMLGSSEDN TALSRALSQL AEVEEKIEQL HQEQANNDFF LLAELLSDYI

    RLLAIVRAAF DQRMKTWQRW QDAQATLQKK REAEARLLWA NKPDKLQQAK

    DEILEWESRV TQYERDFERI STVVRKEVIR FEKEKSKDFK NHVIKYLETL

    LYSQQQLAKY WEAFLPEAKA IS

  • UCSC uc002amv.4; human. [Q13596-1];


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