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Human BCAT2 ELISA Kit

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  • Alternative name

    Human BCAT(m) ELISA Kit;Human placental protein 18 ELISA Kit;Human PP18 ELISA Kit;Human BCATM ELISA Kit;Human BCT2 ELISA Kit;Human ECA40 ELISA Kit;Human BCAM ELISA Kit;Human branched chain amino acid transaminase 2 ELISA Kit;Human branched-chain-amino-acid aminotransferase, mitochondrial ELISA Kit;Human branched chain amino-acid transaminase 2, mitochondrial ELISA Kit;Human branched chain aminotransferase 2, mitochondrial ELISA Kit;
  • Catalog
    E005264
  • species
    Human
  • GeneBCAT2
  • Standard CurveHuman BCAT2 ELISA Kit
  • Other Species Mouse Bcat2 ELISA KitBovine BCAT2 ELISA KitPorcine BCAT2 ELISA KitRat Bcat2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of BCAT2. No significant cross-reactivity or interference between BCAT2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BCAT2 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman BCAT2 ELISA Kit allows for the in vitro quantitative determination of BCAT2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Intended Uses: This BCAT2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human BCAT2. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: BCAT2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-BCAT2 antibody and an BCAT2-HRP conjugate. The assay sample and buffer are incubated together with BCAT2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BCAT2 concentration since BCAT2 from samples and BCAT2-HRP conjugate compete for the anti-BCAT2 antibody binding site. Since the number of sites is limited, as more sites are occupied by BCAT2 from the sample, fewer sites are left to bind BCAT2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BCAT2 concentration in each sample is interpolated from this standard curve.
  • Human Branched-chain-amino-acid aminotransferase, mitochondrial Protein information
  • Uniprot ID BCAT2_HUMAN
  • Uniprot AC O15382; B2RB87; O00269; Q96KG1; Q9BTB6; Q9BUU6;
  • UniGene Hs.512670;
  • GeneID 587
  • KEGG hsa:587;
  • Human Branched-chain-amino-acid aminotransferase, mitochondrial Protein SEQUENCE

  • SEQUENCE 392 AA; 44288 MW; EC07047264B190DA CRC64;

    MAAAALGQIW ARKLLSVPWL LCGPRRYASS SFKAADLQLE MTQKPHKKPG

    PGEPLVFGKT FTDHMLMVEW NDKGWGQPRI QPFQNLTLHP ASSSLHYSLQ

    LFEGMKAFKG KDQQVRLFRP WLNMDRMLRS AMRLCLPSFD KLELLECIRR

    LIEVDKDWVP DAAGTSLYVR PVLIGNEPSL GVSQPTRALL FVILCPVGAY

    FPGGSVTPVS LLADPAFIRA WVGGVGNYKL GGNYGPTVLV QQEALKRGCE

    QVLWLYGPDH QLTEVGTMNI FVYWTHEDGV LELVTPPLNG VILPGVVRQS

    LLDMAQTWGE FRVVERTITM KQLLRALEEG RVREVFGSGT ACQVCPVHRI

    LYKDRNLHIP TMENGPELIL RFQKELKEIQ YGIRAHEWMF PV

  • UCSC uc002pkr.4; human. [O15382-1];


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