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cyclin E Antibody (HE111): sc-248

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Datasheets
  • cyclin E Antibody (HE111) is a mouse monoclonal IgG1 λ, cited in 134 publications, provided at 200 µg/ml
  • raised against recombinant cyclin E of human origin
  • Anti-cyclin E Antibody (HE111) is recommended for detection of cyclin E of mouse, rat and human origin by WB, IP, IF and IHC(P)
  • Anti-cyclin E Antibody (HE111) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to TRITC for IF, IHC(P) and FCM
  • TransCruz reagent for Gel Supershift and ChIP applications (sc-248 X, 200 µg/0.1 ml)
  • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of cyclin E (HE111): sc-248.
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGλ BP-HRP are the preferred secondary detection reagents for cyclin E Antibody (HE111) for WB and IHC(P) applications. These reagents are now offered in bundles with cyclin E Antibody (HE111) (see ordering information below).

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Cyclin E Antibody (HE111) is a mouse monoclonal IgG1 lambda light chain antibody that detects cyclin E of mouse, rat, and human origin through western blotting, immunoprecipitation, immunofluorescence, and immunohistochemistry. Cyclin E (HE111) antibody is offered in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. Cyclin E plays a pivotal role in the regulation of the cell cycle, particularly during the transition from the G1 phase to S phase. Cyclin E partners with the transcription factor E2F to drive the expression of genes necessary for DNA synthesis. The cyclin E/E2F complex is predominantly active during G1 phase, where cyclin E facilitates the expression of S phase-specific proteins, ensuring cells are adequately prepared for DNA replication. Precise regulation of cyclin E levels is crucial, as dysregulation can lead to uncontrolled cell proliferation and is often implicated in various cancers. Cyclin E monoclonal antibody (HE111) serves as a valuable tool for researchers studying cell cycle dynamics and molecular mechanisms underlying cell division and cancer biology.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

cyclin E Antibody (HE111)

sc-248
200 µg/ml
$316.00

cyclin E Antibody (HE111): m-IgG Fc BP-HRP Bundle

sc-551158
200 µg Ab; 10 µg BP
$354.00

cyclin E Antibody (HE111): m-IgGλ BP-HRP Bundle

sc-551157
200 µg Ab; 40 µg BP
$354.00

cyclin E Antibody (HE111): m-IgG1 BP-HRP Bundle

sc-551159
200 µg Ab; 20 µg BP
$354.00

cyclin E Antibody (HE111) X

sc-248 X
200 µg/0.1 ml
$316.00

cyclin E Antibody (HE111) AC

sc-248 AC
500 µg/ml, 25% agarose
$416.00

cyclin E Antibody (HE111) HRP

sc-248 HRP
200 µg/ml
$316.00

cyclin E Antibody (HE111) FITC

sc-248 FITC
200 µg/ml
$330.00

cyclin E Antibody (HE111) PE

sc-248 PE
200 µg/ml
$343.00

cyclin E Antibody (HE111) Alexa Fluor® 488

sc-248 AF488
200 µg/ml
$357.00

cyclin E Antibody (HE111) Alexa Fluor® 546

sc-248 AF546
200 µg/ml
$357.00

cyclin E Antibody (HE111) Alexa Fluor® 594

sc-248 AF594
200 µg/ml
$357.00

cyclin E Antibody (HE111) Alexa Fluor® 647

sc-248 AF647
200 µg/ml
$357.00

cyclin E Antibody (HE111) Alexa Fluor® 680

sc-248 AF680
200 µg/ml
$357.00

cyclin E Antibody (HE111) Alexa Fluor® 790

sc-248 AF790
200 µg/ml
$357.00

cyclin E Antibody (HE111) TRITC

sc-248 TRITC
200 µg/ml
$320.00

Why is CCNE1detecting a 90kD band with this antibody?

Asked by: Ling
Thank you for your question. The protein undergoes phosphorylation.https://www.uniprot.org/uniprotkb/P24864/entry#ptm_processing
Answered by: BlakeJ
Date published: 2024-08-09

Can this antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. We recommend following our recommended protocol, provided below, https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 7
Date published: 2017-01-25
  • y_2025, m_3, d_31, h_7CST
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Rated 5 out of 5 by from a good antibody for WBThis antibody generates a clear signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
Rated 5 out of 5 by from mouse livernice antibody 1:500 dilution, in mouse liver tissue.
Date published: 2017-03-31
Rated 5 out of 5 by from Great IHC resultsTested multiple samples by IHC in paraffin embedded sections with great results!
Date published: 2017-01-17
Rated 5 out of 5 by from Good data with paraffinGood data with paraffin-embedded human ovary tissue. -SCBT Publication Review
Date published: 2015-02-11
Rated 4 out of 5 by from This antibody works well and can be usedThis antibody works well and can be used for IP with MEG-01 samples. -SCBT QC
Date published: 2015-01-29
Rated 5 out of 5 by from WB: Great antibodyWB: Great antibody, good images for publication with human U2OS. -SCBT Publication Review
Date published: 2014-08-20
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cyclin E Antibody (HE111) is rated 4.8 out of 5 by 6.
  • y_2025, m_3, d_31, h_7
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
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