Cdk4 Antibody (DCS-156): sc-53636

Cdk4 Antibody (DCS-156) is a mouse monoclonal IgG1 kappa light chain antibody specifically designed to detect Cyclin-dependent kinase 4 (Cdk4), also known as CDC2-like kinase 4, in mouse, rat, and human samples. Anti-Cdk4 antibody (DCS-156) targets an epitope within amino acids 270-290 of the human Cdk4 protein, ensuring precise recognition in applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Cdk4 is a crucial serine/threonine kinase that plays a pivotal role in regulating the cell cycle, particularly the transition from the G1 to the S phase, by forming complexes with D-type cyclins. The structural domains of Cdk4, including its kinase domain and cyclin-binding regions, are essential for its ability to phosphorylate key substrates like the retinoblastoma protein (Rb), which drives cellular proliferation and ensures proper cell cycle progression. The integrity and proper folding of Cdk4′s structure are vital for interactions with regulatory proteins, such as the inhibitor p16, which modulates activity to maintain controlled cell division. Alterations or mutations in the structure of Cdk4 can disrupt function and are often associated with various cancers, highlighting significance as a target for therapeutic interventions. Understanding the structural dynamics of Cdk4, including activation through phosphorylation and cyclin binding, is fundamental for elucidating role in cell cycle regulation and oncogenesis. Cdk4 (DCS-156) antibody is available in both non-conjugated form and a variety of conjugated formats to accommodate diverse experimental needs, providing researchers with a reliable tool to investigate the structural and functional aspects of Cdk4 in cell cycle dynamics and cancer research.

The preferred Cdk4 antibody is Cdk4 Antibody (DCS-35): sc-23896.