NBK Antibody (H-1): sc-365625

NBK Antibody (H-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects NBK protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). NBK (H-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NBK protein, also known as Bik, plays a crucial role in regulating apoptosis, acting as a pro-apoptotic factor that promotes cell death in response to various stimuli. Unlike other members of the Bcl-2 family, NBK protein does not contain conserved Bcl-2 homology domains typically associated with anti-apoptotic functions. NBK protein shares a critical BH3 motif with Bax, a well-known pro-apoptotic protein, suggesting similar mechanisms of action. NBK′s ability to induce apoptosis is particularly important in cancer, where evasion of programmed cell death is a hallmark of tumorigenesis. Understanding NBK′s interactions and functions can provide insights into potential therapeutic strategies aimed at reactivating apoptotic pathways in cancer cells.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

NBK Antibody (H-1) References:

1. Btf, a novel death-promoting transcriptional repressor that interacts with Bcl-2-related proteins.  |  Kasof, GM., et al. 1999. Mol Cell Biol. 19: 4390-404. PMID: 10330179
2. Caspase-independent induction of apoptosis in human melanoma cells by the proapoptotic Bcl-2-related protein Nbk / Bik.  |  Oppermann, M., et al. 2005. Oncogene. 24: 7369-80. PMID: 16007125
3. A molecular analysis by gene expression profiling reveals Bik/NBK overexpression in sporadic breast tumor samples of Mexican females.  |  García, N., et al. 2005. BMC Cancer. 5: 93. PMID: 16060964
4. Loss of the tissue-specific proapoptotic BH3-only protein Nbk/Bik is a unifying feature of renal cell carcinoma.  |  Sturm, I., et al. 2006. Cell Death Differ. 13: 619-27. PMID: 16322756
5. BCL2 protein is topographically restricted in tissues characterized by apoptotic cell death.  |  Hockenbery, DM., et al. 1991. Proc Natl Acad Sci U S A. 88: 6961-5. PMID: 1871110
6. Deregulated Bcl-2 gene expression selectively prolongs survival of growth factor-deprived hemopoietic cell lines.  |  Nuñez, G., et al. 1990. J Immunol. 144: 3602-10. PMID: 2184193
7. BIK/NBK gene as potential marker of prognostic and therapeutic target in breast cancer patients.  |  López-Muñoz, E., et al. 2012. Clin Transl Oncol. 14: 586-91. PMID: 22855140
8. Cultivation and partial characterization of bovine astrovirus.  |  Aroonprasert, D., et al. 1989. Vet Microbiol. 19: 113-25. PMID: 2495698
9. Cloning the chromosomal breakpoint of t(14;18) human lymphomas: clustering around JH on chromosome 14 and near a transcriptional unit on 18.  |  Bakhshi, A., et al. 1985. Cell. 41: 899-906. PMID: 3924412
10. Bik, a novel death-inducing protein shares a distinct sequence motif with Bcl-2 family proteins and interacts with viral and cellular survival-promoting proteins.  |  Boyd, JM., et al. 1995. Oncogene. 11: 1921-8. PMID: 7478623
11. Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death.  |  Oltvai, ZN., et al. 1993. Cell. 74: 609-19. PMID: 8358790
12. Bcl-2 blocks apoptosis in cells lacking mitochondrial DNA.  |  Jacobson, MD., et al. 1993. Nature. 361: 365-9. PMID: 8381212

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NBK Antibody (H-1) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-NBK Antibody (H-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NBK Antibody (H-1) Specifications

Alternate Product Name: BIK

Product Clone Name: H-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-160

Epitope Target: NBK

Epitope Species: human

Product Reactivity/Detection: NBK

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for NBK Antibody (H-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin