Mpk1 Antibody (F-7): sc-374435

Mpk1 Antibody (F-7) is a mouse monoclonal IgG2a antibody that detects Mpk1 in yeast by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Mpk1, also known as Slt2, is a crucial component of the mitogen-activated protein kinase (MAPK) signaling cascade that plays a vital role in maintaining cell wall integrity in yeast. Mpk1 is primarily located in the cytoplasm and translocates to the nucleus in response to cell wall stress, where Mpk1 regulates gene expression necessary for cell wall remodeling and repair. Proper functioning of Mpk1 is essential, as mutants lacking Mpk1 exhibit severe defects in cell lysis due to compromised cell wall synthesis, leading to impaired growth and survival under stress conditions. Additionally, Mpk1 interacts with various upstream kinases, including Bck1 and Mkk1/Mkk2, which are critical for activation and subsequent signaling pathways. anti-Mpk1 antibody (F-7) is an invaluable tool for researchers studying the MAPK signaling pathway and its implications in yeast biology, providing insights into cellular responses to environmental challenges.

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Mpk1 Antibody (F-7) References:

1. Yeast Mpk1 mitogen-activated protein kinase activates transcription through Swi4/Swi6 by a noncatalytic mechanism that requires upstream signal.  |  Kim, KY., et al. 2008. Mol Cell Biol. 28: 2579-89. PMID: 18268013
2. Yeast Mpk1 cell wall integrity mitogen-activated protein kinase regulates nucleocytoplasmic shuttling of the Swi6 transcriptional regulator.  |  Kim, KY., et al. 2010. Mol Biol Cell. 21: 1609-19. PMID: 20219973
3. Transcriptional reporters for genes activated by cell wall stress through a non-catalytic mechanism involving Mpk1 and SBF.  |  Kim, KY. and Levin, DE. 2010. Yeast. 27: 541-8. PMID: 20641022
4. TORC1 controls G1-S cell cycle transition in yeast via Mpk1 and the greatwall kinase pathway.  |  Moreno-Torres, M., et al. 2015. Nat Commun. 6: 8256. PMID: 26356805
5. Variants of the yeast MAPK Mpk1 are fully functional independently of activation loop phosphorylation.  |  Goshen-Lago, T., et al. 2016. Mol Biol Cell. 27: 2771-83. PMID: 27413009
6. TORC1, Tel1/Mec1, and Mpk1 regulate autophagy induction after DNA damage in budding yeast.  |  Ueda, S., et al. 2019. Cell Signal. 62: 109344. PMID: 31201849
7. The methanol sensor Wsc1 and MAPK Mpk1 suppress degradation of methanol-induced peroxisomes in methylotrophic yeast.  |  Ohsawa, S., et al. 2021. J Cell Sci. 134: PMID: 33771930
8. Hsp90 and phosphorylation of the Slt2(Mpk1) MAP kinase activation loop are essential for catalytic, but not non-catalytic, Slt2-mediated transcription in yeast.  |  Millson, SH., et al. 2022. Cell Stress Chaperones. 27: 295-304. PMID: 35420390
9. Characterization of domains in the yeast MAP kinase Slt2 (Mpk1) required for functional activity and in vivo interaction with protein kinases Mkk1 and Mkk2.  |  Soler, M., et al. 1995. Mol Microbiol. 17: 833-42. PMID: 8596433
10. Role of calcineurin and Mpk1 in regulating the onset of mitosis in budding yeast.  |  Mizunuma, M., et al. 1998. Nature. 392: 303-6. PMID: 9521328

The preferred Mpk1 antibody is Mpk1 Antibody (D-1): sc-374434.