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Cytokeratin 7/13/17/18 Antibody (LdS 23): sc-53265

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Datasheets
  • Cytokeratin 7/13/17/18 Antibody (LdS 23) is a mouse monoclonal IgG1 κ Cytokeratin 7/13/17/18 antibody, cited in 1 publications, provided at 200 µg/ml
  • raised against cytoskeletons from urothelial RT4 cell line of human origin
  • recommended for detection of Cytokeratin 7/13/17/18 of human origin by WB, IP and IF
  • See Cytokeratin 7 (RCK105): sc-23876 for Cytokeratin 7/13/17/18 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cytokeratin 7/13/17/18 Antibody (LdS 23) for WB applications. These reagents are now offered in bundles with Cytokeratin 7/13/17/18 Antibody (LdS 23) (see ordering information below).

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Cytokeratin 7/13/17/18 Antibody (LdS 23) is a mouse monoclonal IgG1 kappa light chain antibody engineered to detect multiple members of the Cytokeratin family, including Cytokeratin 7 (KRT7), Cytokeratin 13 (KRT13), Cytokeratin 17 (KRT17), and Cytokeratin 18 (KRT18). Raised against cytoskeleton preparations from the human urothelial RT4 cell line, anti-Cytokeratin 7/13/17/18 antibody (LdS 23) exhibits strong reactivity with human Cytokeratins 7, 13, 17, and 18, making this product ideal for applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Cytokeratins are essential intermediate filament proteins that provide structural integrity and resilience to epithelial cells, facilitating cellular architecture and tissue stability. Specifically, Cytokeratin 7 interacts with Cytokeratin 8 to form stable filaments, while Cytokeratin 13 pairs with Cytokeratin 4, and Cytokeratin 17 associates with Cytokeratin 5, playing critical roles in epithelial differentiation and repair processes. Cytokeratin 18 partners with Cytokeratin 8 to support cellular structure and dynamics, and these interactions are vital for maintaining epithelial cell function and integrity. Cytokeratin 7/13/17/18 (LdS 23) antibody′s ability to simultaneously detect these cytokeratins allows researchers to study complex protein interactions and the intricate network of the cytoskeleton in epithelial biology. This multiplex detection is particularly important for investigating molecular mechanisms underlying epithelial differentiation, tissue specialization, and the pathogenesis of various epithelial-derived cancers. Anti-Cytokeratin 7/13/17/18 antibody (LdS 23) provides comprehensive insights into epithelial cell architecture and pathology, enhancing diagnostic accuracy and facilitating advanced research in cell biology, pathology, and oncology.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Cytokeratin 7/13/17/18 Antibody (LdS 23)

sc-53265
200 µg/ml
$316.00

Cytokeratin 7/13/17/18 Antibody (LdS 23): m-IgG Fc BP-HRP Bundle

sc-536921
200 µg Ab; 10 µg BP
$354.00

Cytokeratin 7/13/17/18 Antibody (LdS 23): m-IgGκ BP-HRP Bundle

sc-534096
200 µg Ab; 40 µg BP
$354.00

Cytokeratin 7/13/17/18 Antibody (LdS 23): m-IgG1 BP-HRP Bundle

sc-544901
200 µg Ab; 20 µg BP
$354.00

What is the recommended starting dilution for Western blot for Cytokeratin 7/13/17/18 (LdS 23): sc-53265 monoclonal antibody?

Asked by: Trav11
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-53265.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from Worked with transfected lysate by WB withWorked with transfected lysate by WB with some background -SCBT QC
Date published: 2015-04-13
Rated 5 out of 5 by from Good specificity and signal strength forGood specificity and signal strength for WB in HeLa and A549 whole cell lysates. -SCBT QC
Date published: 2013-07-25
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Cytokeratin 7/13/17/18 Antibody (LdS 23) is rated 4.5 out of 5 by 2.
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