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alpha Tubulin Antibody (TU-02): sc-8035

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Datasheets
  • alpha Tubulin Antibody (TU-02) is a mouse monoclonal IgM κ, cited in 1,490 publications, provided at 200 µg/ml
  • raised against amino acids 1-451 representing full length α Tubulin of porcine origin
  • Anti-alpha Tubulin Antibody (TU-02) is recommended for detection of α Tubulin of mouse, rat, human and porcine origin by WB, IP, IF, IHC(P), FCM and ELISA
  • Anti-alpha Tubulin Antibody (TU-02) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • available conjugated to agarose (sc-8035 AC), 500 µg/ml, 25% agarose, for IP; and to phycoerythrin (sc-8035 PE), 200 µg/ml, for IF, IHC(P) and FCM
  • also available conjugated to either TRITC or Alexa Fluor® 405 for IF, IHC(P) and FCM
  • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of alpha Tubulin (TU-02): sc-8035.
  • m-IgGκ BP-HRP is the preferred secondary detection reagent for alpha Tubulin Antibody (TU-02) for WB and IHC(P) applications. This reagent is now offered in a bundle with alpha Tubulin Antibody (TU-02) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

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α Tubulin Antibody (TU-02) is a mouse monoclonal IgM kappa light chain antibody, raised against the full-length α Tubulin protein (amino acids 1-451) from porcine origin. α Tubulin monoclonal antibody (TU-02) detects α Tubulin from a variety of species, including mouse, rat, human, and porcine, and can be used in techniques such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). α Tubulin pairs with β Tubulin to form heterodimers that assemble into microtubules, structures essential for cell shape, intracellular transport, and mitosis. These microtubules are particularly important during cell division, where they form the mitotic spindle, guiding chromosome segregation. α Tubulin is located primarily in the cytoplasm, forming the building blocks of microtubules that extend throughout the cell. α Tubulin′s vital role in maintaining cellular integrity and supporting microtubule dynamics makes anti-α Tubulin antibody (TU-02) a powerful tool for studying the cytoskeleton and related cellular processes. α Tubulin (TU-02) antibody is available in both non-conjugated and conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and Alexa Fluor® conjugates, providing flexibility for various experimental needs.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

alpha Tubulin Antibody (TU-02) References:

  1. Diversity of α-tubulin transcripts in Lolium rigidum.  |  Chen, J., et al. 2021. Pest Manag Sci. 77: 970-977. PMID: 32991064
  2. SUMOylation of α-tubulin is a novel modification regulating microtubule dynamics.  |  Feng, W., et al. 2021. J Mol Cell Biol. 13: 91-103. PMID: 33394042
  3. Vasohibin-1 has α-tubulin detyrosinating activity in glomerular podocytes.  |  Mifune, T., et al. 2022. Biochem Biophys Res Commun. 599: 93-99. PMID: 35180473
  4. Tubulin expression and modification in heart failure with preserved ejection fraction (HFpEF).  |  Schulz, L., et al. 2022. Sci Rep. 12: 15734. PMID: 36131110
  5. Alpha-tubulin relocalization is involved in calcium oxalate-induced tight junction disruption in renal epithelial cells.  |  Hadpech, S., et al. 2022. Chem Biol Interact. 368: 110236. PMID: 36349591
  6. Tubulin isotype regulation maintains asymmetric requirement for α-tubulin over β-tubulin.  |  Wethekam, LC. and Moore, JK. 2023. J Cell Biol. 222: PMID: 36719400
  7. Integrated regulation of tubulin tyrosination and microtubule stability by human α-tubulin isotypes.  |  Fu, G., et al. 2023. Cell Rep. 42: 112653. PMID: 37379209
  8. Acetylated α-Tubulin and α-Synuclein: Physiological Interplay and Contribution to α-Synuclein Oligomerization.  |  Calogero, AM., et al. 2023. Int J Mol Sci. 24: PMID: 37569662
  9. Suppression of alpha-tubulin acetylation potentiates therapeutic efficacy of Eribulin in liver cancer.  |  Zhong, Y., et al. 2023. Am J Cancer Res. 13: 5698-5718. PMID: 38058833
  10. Metabolic regulation of cytoskeleton functions by HDAC6-catalyzed α-tubulin lactylation.  |  Sun, S., et al. 2024. Nat Commun. 15: 8377. PMID: 39333081

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

alpha Tubulin Antibody (TU-02)

sc-8035
200 µg/ml
$316.00

alpha Tubulin Antibody (TU-02): m-IgGκ BP-HRP Bundle

sc-550728
200 µg Ab; 40 µg BP
$354.00

alpha Tubulin Antibody (TU-02) AC

sc-8035 AC
500 µg/ml, 25% agarose
$416.00

alpha Tubulin Antibody (TU-02) HRP

sc-8035 HRP
200 µg/ml
$316.00

alpha Tubulin Antibody (TU-02) FITC

sc-8035 FITC
200 µg/ml
$330.00

alpha Tubulin Antibody (TU-02) PE

sc-8035 PE
200 µg/ml
$343.00

alpha Tubulin Antibody (TU-02) Alexa Fluor® 488

sc-8035 AF488
200 µg/ml
$357.00

alpha Tubulin Antibody (TU-02) Alexa Fluor® 546

sc-8035 AF546
200 µg/ml
$357.00

alpha Tubulin Antibody (TU-02) Alexa Fluor® 594

sc-8035 AF594
200 µg/ml
$357.00

alpha Tubulin Antibody (TU-02) Alexa Fluor® 647

sc-8035 AF647
200 µg/ml
$357.00

alpha Tubulin Antibody (TU-02) Alexa Fluor® 680

sc-8035 AF680
200 µg/ml
$357.00

alpha Tubulin Antibody (TU-02) Alexa Fluor® 790

sc-8035 AF790
200 µg/ml
$357.00

alpha Tubulin Antibody (TU-02) TRITC

sc-8035 TRITC
200 µg/ml
$320.00

alpha Tubulin Antibody (TU-02) Alexa Fluor® 405

sc-8035 AF405
200 µg/ml
$357.00

i have used SC-8035 primary antibody with sc-2064 secondary antibody. it seems sc-2064 is no more available. what secondary i should use for western blotting.

Asked by: Nandita
Thank you for your question. We recommend using an anti-mouse IgM secondary. Santa Cruz Biotechnology do not offer IgM secondaries .
Answered by: BlakeJ
Date published: 2022-11-14

LOT# D0921 did not work for WB against human and mouse whole cell lysates (C2C12, 3T3-L1, and HEK293 cells). I think it may have a problem with this LOT# product. Would you check whether is it okay? 

Asked by: Stiger22
Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Service SH
Date published: 2022-07-04

Is this antibody recommended for using as a loading control for western blotting of both Mouse and human samples?

Asked by: Islam
Yes, this antibody can be used for this purpose.
Answered by: Dallas Tech Service
Date published: 2021-06-18

Hi, I have purchased this antibody a few weeks ago. Initially, the band took so long to appear at 55kDa. The second time I tried, another band appear at 45kDa which is more intense. The band at 55kDa took more than 3 hours to develop

Asked by: Siti Khadijah
Thank you for your message. Please get in touch with our technical service team directly so we can troubleshoot your experiment.
Answered by: Tech Support Europe
Date published: 2020-08-05

Liebes Santa Cruz Team, besteht die Möglichkeit ein Testsample oder kleineres Muster von α Tubulin Antikörper (TU-02): sc-8035 mit FITC oder ähnlichem konjugiert zu erhalten? In welcher Verdünnung wird der AK für die IF eingesetzt? Vielen dank

Asked by: AKKlein
Vielen Dank für Ihre Anfrage. Wir bieten nur unkonjugierte Primärantikörper als kostenlose Samples an. Sie können auch unsere empfohlenen sekundären Bindeproteine als Samples erhalten, wie z.B. m-IgGκ BP-FITC: sc-516140 oder m-IgGκ BP-CFL 488: sc-516176. Kontaktieren Sie gerne unseren technischen Service um die Lieferung entsprechender Samples zu arrangieren.
Answered by: Tech Support Europe
Date published: 2019-06-14

Has this antibody been used on mouse brain sections?

Asked by: Alex3
Thank you for your question. This antibody is fine for mouse tissue sections, so it should be fine for your mouse brain samples. If you have any further questions, please reach out to Technical support at scbt@scbt.com, or via phone at 800-457-3801, ext 2 or via chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2017-12-04

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: Cweed
Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. SC-8035 is currently available conjugated to FITC, AF488, AF647.
Answered by: TechService7
Date published: 2016-12-14

How can this mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: Cweed
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. SC-8035 is currently available conjugated to AF488, AF647, and FITC.
Answered by: TechService7
Date published: 2016-12-14
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Rated 4 out of 5 by from Buon anticorpoHo testato l'ab in immunofluorescenza su neurone e ho ottenuto un ottimo risultato con diluizione 1:250.
Date published: 2024-07-22
Rated 5 out of 5 by from Great for WBThe antibody is good for western blot. The band is single and clear.
Date published: 2024-03-19
Rated 5 out of 5 by from Muy buen reconocimiento de la proteínaGood antibody for tubulin detection in WB. It gives a strong signal.
Date published: 2023-10-03
Rated 5 out of 5 by from sc-8035We use this antibody in western blot, and it works well.
Date published: 2023-07-07
Rated 4 out of 5 by from Sharp and single bandI am so happy to use this antibody for paper publication.
Date published: 2023-03-14
Rated 5 out of 5 by from Excellent antibody!Good for both immunoblotting and immunofluorescence of human proteins.
Date published: 2020-10-09
Rated 5 out of 5 by from 很好用帮客户买的,客户评价很好用,条带清晰。没有杂带。会继续回购的。对课题很有帮助。希望多一点折扣。经费在燃烧
Date published: 2020-09-18
Rated 5 out of 5 by from Good antibody for western blottinga-Tubulin Antibody (TU-02) is good quality for western blotting (dilute factor: 1:5000)
Date published: 2020-05-25
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alpha Tubulin Antibody (TU-02) is rated 4.7 out of 5 by 79.
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