IgM Antibody (2C12-3): sc-69920

IgM Antibody (2C12-3) is a mouse monoclonal IgG2b antibody that detects Immunoglobulin M (IgM) in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). IgM is the largest circulating antibody molecule in humans, consisting of a heavy chain (µ-chain) and a light chain (κ- or λ-chain), forming a pentameric structure that provides ten binding sites, although not all can be utilized simultaneously due to steric hindrance. The unique structure of IgM, particularly its polymeric nature, is crucial for high avidity and effectiveness in activating the complement system, which plays a vital role in the immune response by marking pathogens for destruction. Additionally, IgM is primarily found in serum but also functions as a secretory immunoglobulin due to the presence of the J chain, allowing involvement in mucosal immunity. As the first immunoglobulin expressed by mature B cells, IgM is typically the first line of defense during an initial infection, appearing early in the immune response and serving as a critical marker for diagnosing various infectious diseases. Anti-IgM antibody (2C12-3) is an essential tool for researchers studying immune responses and the role of antibodies in human health.

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IgM Antibody (2C12-3) References:

1. IgM bullous disease associated with IgM gammopathy: a report of two cases and review.  |  le Roux-Villet, C., et al. 2004. Br J Dermatol. 150: 392-4. PMID: 14996131
2. Suppression of IgM Proteolysis by Conformational Stabilization Through Excipients.  |  Mueller, M., et al. 2015. Sci Pharm. 83: 401-10. PMID: 26839826
3. Early ovariectomy reveals the germline encoding of natural anti-A- and Tn-cross-reactive immunoglobulin M (IgM) arising from developmental O-GalNAc glycosylations. (Germline-encoded natural anti-A/Tn cross-reactive IgM).  |  Arend, P. 2017. Cancer Med. 6: 1601-1613. PMID: 28580709
4. Transient pentameric IgM fulfill biological function-Effect of expression host and transfection on IgM properties.  |  Hennicke, J., et al. 2020. PLoS One. 15: e0229992. PMID: 32163462
5. Differential Expression of IgM and IgD Discriminates Two Subpopulations of Human Circulating IgM+IgD+CD27+ B Cells That Differ Phenotypically, Functionally, and Genetically.  |  Bautista, D., et al. 2020. Front Immunol. 11: 736. PMID: 32435242
6. Plasmodium falciparum-specific IgM B cells dominate in children, expand with malaria, and produce functional IgM.  |  Hopp, CS., et al. 2021. J Exp Med. 218: PMID: 33661303
7. Teleost IgD+IgM- B cells in gills and skin have a plasmablast profile, but functionally and phenotypically differ from IgM+IgD- B cells in these sites.  |  Herranz-Jusdado, JG., et al. 2023. iScience. 26: 107434. PMID: 37593459
8. Reference values for CSF-IgM, CSF-IgM/S-IgM ratio and IgM index, and its application to patients with multiple sclerosis and aseptic meningoencephalitis.  |  Forsberg, P., et al. 1984. Scand J Clin Lab Invest. 44: 7-12. PMID: 6701452
9. Local synthesis of IgM and IgM rheumatoid factor in rheumatoid pleuritis.  |  Halla, JT., et al. 1983. J Rheumatol. 10: 204-9. PMID: 6864674
10. IgM auto-antibody thrombocytopenia simulating pseudothrombocytopenia.  |  Duschek, EJ., et al. 1997. Eur J Haematol. 58: 295-6. PMID: 9186545

The preferred IgM antibody is IgM Antibody (R1/69): sc-53347.