CD8 Antibody (UCH-T4): sc-1181

CD8 Antibody (UCH-T4) is a mouse monoclonal IgG2a kappa light chain antibody that detects the CD8 protein of human origin by immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). Anti-CD8 antibody (UCH-T4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CD8, also known as Leu 2 or T8, is a critical cell surface glycoprotein that forms a two-chain complex (either αα or αβ) and plays a vital role in the immune response by binding to class I major histocompatibility complex (MHC) molecules presented by antigen-presenting cells (APCs). The primary function of CD8 is to enhance antigen recognition by the T cell receptor (TCR), thereby strengthening the interactions between the TCR and the antigen, which is essential for effective T cell activation and response. Additionally, CD8-expressing T cells are crucial in maintaining immune surveillance and may help control viral infections, such as HIV, by keeping viral loads in check. The ability of CD8 to facilitate TCR signaling and its involvement in the immune response underscores its importance in both adaptive immunity and potential therapeutic applications in immunology and oncology.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CD8 Antibody (UCH-T4) References:

1. The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation.  |  Janeway, CA. 1992. Annu Rev Immunol. 10: 645-74. PMID: 1534242
2. The immunobiology of T cells with invariant gamma delta antigen receptors.  |  Allison, JP. and Havran, WL. 1991. Annu Rev Immunol. 9: 679-705. PMID: 1832874
3. CD4 and CD8 act as co-receptors during thymic selection of the T cell repertoire.  |  Zúñiga-Pflücker, JC., et al. 1991. Semin Immunol. 3: 167-75. PMID: 1909594
4. CD4 and CD8 recognition of class II and class I molecules of the major histocompatibility complex.  |  Fleury, SG., et al. 1991. Semin Immunol. 3: 177-85. PMID: 1909595
5. Structure and expression of the gene encoding CD8 alpha chain (Leu-2/T8).  |  Nakayama, K., et al. 1989. Immunogenetics. 30: 393-7. PMID: 2509342
6. CD8+ T cells regulate tumour ferroptosis during cancer immunotherapy.  |  Wang, W., et al. 2019. Nature. 569: 270-274. PMID: 31043744
7. CD8+ T cell differentiation and dysfunction in cancer.  |  Philip, M. and Schietinger, A. 2022. Nat Rev Immunol. 22: 209-223. PMID: 34253904
8. CD8+ T Cell Exhaustion in Cancer.  |  Dolina, JS., et al. 2021. Front Immunol. 12: 715234. PMID: 34354714
9. HIV-specific CD8+ T-cell immune responses and viral replication.  |  Buseyne, F. and Rivière, Y. 1993. AIDS. 7 Suppl 2: S81-5. PMID: 7909230
10. T cell receptor interaction with peptide/major histocompatibility complex (MHC) and superantigen/MHC ligands is dominated by antigen.  |  Ehrich, EW., et al. 1993. J Exp Med. 178: 713-22. PMID: 8393480
11. Distinct roles for CD4 and CD8 as co-receptors in antigen receptor signalling.  |  Julius, M., et al. 1993. Immunol Today. 14: 177-83. PMID: 8499078
12. A novel leukocyte adhesion deficiency caused by expressed but nonfunctional beta2 integrins Mac-1 and LFA-1.  |  Hogg, N., et al. 1999. J Clin Invest. 103: 97-106. PMID: 9884339

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD8 Antibody (UCH-T4) has 18 citations in a variety of scientific publications.

Primary antibodies like Anti-CD8 Antibody (UCH-T4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD8 Antibody (UCH-T4) Specifications

Alternate Product Name: CD8

Product Clone Name: UCH-T4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: CD8

Epitope Species: human

Product Reactivity/Detection: CD8

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 18 citations in various scientific publications

Available Conjugates/Forms for CD8 Antibody (UCH-T4): AC: Agarose, AF405: Alexa Fluor^® 405, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin