CD45 Antibody (30-F11): sc-53665

CD45 Antibody (30-F11) is a rat monoclonal IgG2b light chain antibody developed to recognize CD45, also known as Protein Tyrosine Phosphatase Receptor Type C (PTPRC). CD45 is a crucial transmembrane glycoprotein expressed on the surface of all nucleated hematopoietic cells in mice, playing a fundamental role in regulating immune cell signaling and activation. Functioning as a tyrosine phosphatase, CD45 modulates key signal transduction pathways by dephosphorylating tyrosine residues on Src family kinases such as Lck and Fyn, as well as on Syk family kinases like ZAP-70. These interactions are essential for the proper functioning of T and B cell receptors, influencing lymphocyte development, activation, and overall immune responses. The ability of CD45 to regulate these signaling pathways is critical for maintaining immune system homeostasis and ensuring effective immune responses against pathogens. Anti-CD45 antibody (30-F11) is raised against thymus and spleen tissues of mouse origin, making CD45 (30-F11) antibody effective for various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). By targeting CD45, anti-CD45 antibody (30-F11) facilitates the study of immune cell populations and their activation states, providing valuable insights into immune system dynamics and the mechanisms underlying immune-related diseases. CD45 monoclonal antibody (30-F11) is an indispensable tool for both research and diagnostic purposes in immunology and hematology, supporting advancements in understanding immune responses and the development of targeted therapies for immune disorders.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD45 Antibody (30-F11) has 29 citations in a variety of scientific publications.

Primary antibodies like Anti-CD45 Antibody (30-F11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD45 Antibody (30-F11) Specifications

Alternate Product Name: PTPRC

Product Clone Name: 30-F11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b

Amino Acids:

Epitope Target: CD45

Epitope Species: mouse

Product Reactivity/Detection: CD45

Species Reactivity/Detection: mouse

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG_2b in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 29 citations in various scientific publications

Available Conjugates/Forms for CD45 Antibody (30-F11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred CD45 antibody is CD45 Antibody (35-Z6): sc-1178.