CD33 Antibody (B-9): sc-374450
- CD33 Antibody (B-9) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
- raised against amino acids 231-340 mapping near the C-terminus of CD33 of human origin
- CD33 Antibody (B-9) is recommended for detection of precursor and mature forms of CD33 of human origin by WB, IP, IF and ELISA; may cross-react with Siglec-5
- Anti-CD33 Antibody (B-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD33 (B-9): sc-374450.
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for CD33 Antibody (B-9) for WB applications. These reagents are now offered in bundles with CD33 Antibody (B-9) (see ordering information below).
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SEE ALSO...
CD33 Antibody (B-9) is a mouse monoclonal IgG2a kappa light chain antibody that detects CD33 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CD33 (B-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CD33 is a type I transmembrane glycoprotein primarily located on the surface of granulocyte and macrophage precursors in the bone marrow, while being absent from pluripotent stem cells. CD33 expression on monocytes in peripheral blood makes this protein a crucial marker for distinguishing myelogenous leukemia cells from lymphoid or erythroid leukemias. CD33 may function as a sialic acid-dependent cell adhesion molecule, playing a significant role in mediating cell-cell interactions and influencing immune responses. Understanding CD33 function is vital, as this protein can impact the development of therapeutic strategies targeting myeloid malignancies and enhance our comprehension of immune system regulation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CD33 Antibody (B-9) References:
- SIGLEC-3 (CD33) serves as an immune checkpoint receptor for HBV infection. | Tsai, TY., et al. 2021. J Clin Invest. 131: PMID: 34060491
- Discovery of Small-Molecule CD33 Pre-mRNA Splicing Modulators. | Chappie, TA., et al. 2022. ACS Med Chem Lett. 13: 55-62. PMID: 35059124
- Alzheimer's Disease-Associated Alternative Splicing of CD33 Is Regulated by the HNRNPA Family Proteins. | Komuro, R., et al. 2023. Cells. 12: PMID: 36831269
- Multiplex Base Editing to Protect from CD33-Directed Therapy: Implications for Immune and Gene Therapy. | Borot, F., et al. 2023. bioRxiv.. PMID: 36865281
- CD33 as a leukocyte-associated marker expressed on human spermatozoa. | Sereshki, N., et al. 2023. BMC Res Notes. 16: 57. PMID: 37081561
- Efficient long-term multilineage engraftment of CD33-edited hematopoietic stem/progenitor cells in nonhuman primates. | Petty, NE., et al. 2023. Mol Ther Methods Clin Dev. 31: 101121. PMID: 37868209
- HuM195 and its single-chain variable fragment increase Aβ phagocytosis in microglia via elimination of CD33 inhibitory signaling. | Wong, E., et al. 2024. Mol Psychiatry. 29: 2084-2094. PMID: 38383769
- CD33 and SHP-1/PTPN6 Interaction in Alzheimer's Disease. | Beckers, L., et al. 2024. Genes (Basel). 15: PMID: 39336795
- CRISPR/Cas9 editing of NKG2A improves the efficacy of primary CD33-directed chimeric antigen receptor natural killer cells. | Bexte, T., et al. 2024. Nat Commun. 15: 8439. PMID: 39349459
- CD70 CAR T cells secreting an anti-CD33/anti-CD3 dual targeting antibody overcome antigen heterogeneity in AML. | Silva, H., et al. 2024. Blood.. PMID: 39571145
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD33 Antibody (B-9) | sc-374450 | 200 µg/ml | $316.00 | |||
CD33 Antibody (B-9): m-IgG Fc BP-HRP Bundle | sc-525966 | 200 µg Ab; 10 µg BP | $354.00 | |||
CD33 Antibody (B-9): m-IgG2a BP-HRP Bundle | sc-546471 | 200 µg Ab; 10 µg BP | $354.00 | |||
CD33 Antibody (B-9) AC | sc-374450 AC | 500 µg/ml, 25% agarose | $416.00 | |||
CD33 Antibody (B-9) HRP | sc-374450 HRP | 200 µg/ml | $316.00 | |||
CD33 Antibody (B-9) FITC | sc-374450 FITC | 200 µg/ml | $330.00 | |||
CD33 Antibody (B-9) PE | sc-374450 PE | 200 µg/ml | $343.00 | |||
CD33 Antibody (B-9) Alexa Fluor® 488 | sc-374450 AF488 | 200 µg/ml | $357.00 | |||
CD33 Antibody (B-9) Alexa Fluor® 546 | sc-374450 AF546 | 200 µg/ml | $357.00 | |||
CD33 Antibody (B-9) Alexa Fluor® 594 | sc-374450 AF594 | 200 µg/ml | $357.00 | |||
CD33 Antibody (B-9) Alexa Fluor® 647 | sc-374450 AF647 | 200 µg/ml | $357.00 | |||
CD33 Antibody (B-9) Alexa Fluor® 680 | sc-374450 AF680 | 200 µg/ml | $357.00 | |||
CD33 Antibody (B-9) Alexa Fluor® 790 | sc-374450 AF790 | 200 µg/ml | $357.00 |