NF-L Antibody (3F285): sc-71678

NF-L Antibody (3F285) is a mouse monoclonal IgG1 antibody that detects neurofilament light polypeptide (NF-L) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). NF-L is a crucial component of the neuronal cytoskeleton, playing a significant role in maintaining the structural integrity of neurons. NF-L (3F285) antibody targets proteins primarily located in the axons of neurons, where NF-L contributes to the regulation of axon caliber and overall stability of the neuronal architecture. Proper functioning of NF-L is vital for neuronal health, as alterations in expression or phosphorylation status can lead to neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS), Parkinson′s disease, and Alzheimer′s disease. The dynamic nature of neurofilaments, which includes their ability to interact with other cytoskeletal elements like microtubules and actin filaments, underscores their importance in neuronal function and signaling. Anti-NF-L antibody (3F285) serves as an essential tool for researchers investigating the role of neurofilaments in health and disease, providing insights into the molecular mechanisms underlying neurodegeneration and potential therapeutic targets.

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NF-L Antibody (3F285) References:

1. Overexpression of neurofilament subunit NF-L and NF-H extends survival of a mouse model for amyotrophic lateral sclerosis.  |  Kong, J. and Xu, Z. 2000. Neurosci Lett. 281: 72-4. PMID: 10686419
2. A novel NF-L mutation Pro22Ser is associated with CMT2 in a large Slovenian family.  |  Georgiou, DM., et al. 2002. Neurogenetics. 4: 93-6. PMID: 12481988
3. Expression of rat neurofilament proteins NF-L and NF-M in transfected non-neuronal cells.  |  Chin, SS. and Liem, RK. 1989. Eur J Cell Biol. 50: 475-90. PMID: 2516804
4. Microbiome-Derived Lipopolysaccharide (LPS) Selectively Inhibits Neurofilament Light Chain (NF-L) Gene Expression in Human Neuronal-Glial (HNG) Cells in Primary Culture.  |  Lukiw, WJ., et al. 2018. Front Neurosci. 12: 896. PMID: 30568571
5. Simultaneous alterations of oligodendrocyte-specific CNP, astrocyte-specific AQP4 and neuronal NF-L demarcate ischemic tissue after experimental stroke in mice.  |  Mages, B., et al. 2019. Neurosci Lett. 711: 134405. PMID: 31374325
6. Cytoskeletal neurofilament gene expression in brain tissue from Alzheimer's disease patients. I. Decrease in NF-L and NF-M message.  |  Kittur, S., et al. 1994. J Geriatr Psychiatry Neurol. 7: 153-8. PMID: 7522458
7. Characterization of the rat light neurofilament (NF-L) gene promoter and identification of NGF and cAMP responsive regions.  |  Reeben, M., et al. 1995. J Neurosci Res. 40: 177-88. PMID: 7745611
8. The rod domain of NF-L determines neurofilament architecture, whereas the end domains specify filament assembly and network formation.  |  Heins, S., et al. 1993. J Cell Biol. 123: 1517-33. PMID: 8253847
9. Assembly properties of amino- and carboxyl-terminally truncated neurofilament NF-H proteins with NF-L and NF-M in the presence and absence of vimentin.  |  Sun, D., et al. 1997. J Neurochem. 68: 917-26. PMID: 9048736
10. Transient expression of the neurofilament proteins NF-L and NF-M by Schwann cells is regulated by axonal contact.  |  Fabrizi, C., et al. 1997. J Neurosci Res. 50: 291-9. PMID: 9373038

The preferred NF-L antibody is NF-L Antibody (8A1): sc-20012.