NF-H Antibody (H-5): sc-133237
- NF-H Antibody (H-5) is a mouse monoclonal IgG2b κ, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 1-100 mapping at the N-terminus of NF-H of human origin
- recommended for detection of NF-H of human origin by WB, IP, IF and ELISA
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for NF-H Antibody (H-5) for WB applications. These reagents are now offered in bundles with NF-H Antibody (H-5) (see ordering information below).
NF-H Antibody (H-5) is a mouse monoclonal IgG2b light chain antibody that detects neurofilament-H (NF-H) in human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NF-H is a crucial component of the neuronal cytoskeleton, belonging to the intermediate filament family, and plays a significant role in maintaining the structural integrity of neurons. NF-H′s dynamic nature is underscored by numerous phosphorylation sites, which are targeted by various protein kinases, including protein kinase A, protein kinase C, and cyclin-dependent kinase 5. These modifications are essential for regulating neurofilament assembly and disassembly, thereby influencing axon caliber and overall neuronal function. Alterations in NF-H phosphorylation and metabolism are often linked to neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS), Parkinson′s disease, and Alzheimer′s disease, making NF-H a valuable biomarker for studying these conditions. Anti-NF-H antibody (H-5) provides researchers with a powerful tool for investigating the role of neurofilaments in neuronal health and disease.
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NF-H Antibody (H-5) References:
- Selective changes in the neurofilament and microtubule cytoskeleton of NF-H/LacZ mice. | Riederer, IM., et al. 2003. J Neurosci Res. 71: 196-207. PMID: 12503082
- Peripherin-mediated death of motor neurons rescued by overexpression of neurofilament NF-H proteins. | Beaulieu, JM. and Julien, JP. 2003. J Neurochem. 85: 248-56. PMID: 12641746
- A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. | Harris, J., et al. 1991. J Neurosci Res. 30: 47-62. PMID: 1724473
- Monoclonal antibodies to the heavy neurofilament subunit (NF-H) of Torpedo cholinergic neurons. | Faigon, M., et al. 1991. J Neurosci Res. 29: 490-8. PMID: 1791640
- Expression of the neurofilament protein NF-H in L cells. | Soifer, D., et al. 1991. J Neurosci Res. 30: 63-71. PMID: 1795408
- In situ localization of NF-H neurofilament subunit mRNAs in rat brain. | Roussel, G., et al. 1991. Dev Neurosci. 13: 98-103. PMID: 2070748
- Varying degrees of phosphorylation determine microheterogeneity of the heavy neurofilament polypeptide (Nf-H). | Goldstein, ME., et al. 1987. J Neuroimmunol. 14: 135-48. PMID: 2434525
- Isolation of a cDNA for the rat heavy neurofilament polypeptide (NF-H). | Robinson, PA., et al. 1986. FEBS Lett. 209: 203-5. PMID: 2878828
- The structure of the largest murine neurofilament protein (NF-H) as revealed by cDNA and genomic sequences. | Shneidman, PS., et al. 1988. Brain Res. 464: 217-31. PMID: 3145094
- Differential dynamics of neurofilament-H protein and neurofilament-L protein in neurons. | Takeda, S., et al. 1994. J Cell Biol. 127: 173-85. PMID: 7929561
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NF-H Antibody (H-5) | sc-133237 | 200 µg/ml | $316.00 | |||
NF-H Antibody (H-5): m-IgGκ BP-HRP Bundle | sc-534606 | 200 µg Ab; 40 µg BP | $354.00 | |||
NF-H Antibody (H-5): m-IgG2b BP-HRP Bundle | sc-549270 | 200 µg Ab; 10 µg BP | $354.00 |