RGL1 Antibody (G-2): sc-377170
- RGL1 Antibody (G-2) is a mouse monoclonal IgG1 κ RGL1 antibody provided at 200 µg/ml
- raised against amino acids 501-588 mapping within an internal region of RGL1 of human origin
- RGL1 Antibody (G-2) is recommended for detection of RGL1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-RGL1 Antibody (G-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of RGL1 (G-2): sc-377170.
- m-IgG1 BP-HRP is the preferred secondary detection reagent for RGL1 Antibody (G-2) for WB applications. This reagent is now offered in a bundle with RGL1 Antibody (G-2) (see ordering information below).
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RGL1 Antibody (G-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects RGL1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RGL1 (G-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RGL1, also known as RalGDS-like 1, is a 768 amino acid guanine nucleotide exchange factor (GEF) that plays a crucial role in the activation of Ral, a key player in oncogenic Ras-induced cell transformation. RGL1 is predominantly expressed in the brain, heart, spleen, kidney, and testis, highlighting its importance in various physiological processes. Structurally, RGL1 features an N-terminal Ras-GEF domain and a C-terminal Ras-interacting domain, which allows RGL1 to interact with the GTP-bound form of Ras through its effector loop, thereby facilitating downstream signaling pathways. RGL1′s involvement in Ras and Ral signaling is significant, as dysregulation of these pathways is often associated with cancer progression. Additionally, RGL1 has two isoforms resulting from alternative splicing, which may contribute to functional diversity and regulatory mechanisms in cellular contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RGL1 Antibody (G-2) References:
- Double-mutant analysis of the interaction of Ras with the Ras-binding domain of RGL. | Shirouzu, M., et al. 1999. Biochemistry. 38: 5103-10. PMID: 10213614
- The human RGL (RalGDS-like) gene: cloning, expression analysis and genomic organization. | Sood, R., et al. 2000. Biochim Biophys Acta. 1491: 285-8. PMID: 10760592
- Arabidopsis RGL1 encodes a negative regulator of gibberellin responses. | Wen, CK. and Chang, C. 2002. Plant Cell. 14: 87-100. PMID: 11826301
- RalGDS is required for tumor formation in a model of skin carcinogenesis. | González-García, A., et al. 2005. Cancer Cell. 7: 219-26. PMID: 15766660
- The merlin tumor suppressor interacts with Ral guanine nucleotide dissociation stimulator and inhibits its activity. | Ryu, CH., et al. 2005. Oncogene. 24: 5355-64. PMID: 16007223
- Transgenic modification of gai or rgl1 causes dwarfing and alters gibberellins, root growth, and metabolite profiles in Populus. | Busov, V., et al. 2006. Planta. 224: 288-99. PMID: 16404575
- The consensus coding sequences of human breast and colorectal cancers. | Sjöblom, T., et al. 2006. Science. 314: 268-74. PMID: 16959974
- RalGDS-like factor (Rlf) is a novel Ras and Rap 1A-associating protein. | Wolthuis, RM., et al. 1996. Oncogene. 13: 353-62. PMID: 8710374
- Characterization of Ral GDP dissociation stimulator-like (RGL) activities to regulate c-fos promoter and the GDP/GTP exchange of Ral. | Murai, H., et al. 1997. J Biol Chem. 272: 10483-90. PMID: 9099691
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RGL1 Antibody (G-2) | sc-377170 | 200 µg/ml | $316.00 | |||
RGL1 Antibody (G-2): m-IgG1 BP-HRP Bundle | sc-531730 | 200 µg Ab; 20 µg BP | $354.00 | |||
RGL1 Antibody (G-2) AC | sc-377170 AC | 500 µg/ml, 25% agarose | $416.00 | |||
RGL1 Antibody (G-2) HRP | sc-377170 HRP | 200 µg/ml | $316.00 | |||
RGL1 Antibody (G-2) FITC | sc-377170 FITC | 200 µg/ml | $330.00 | |||
RGL1 Antibody (G-2) PE | sc-377170 PE | 200 µg/ml | $343.00 | |||
RGL1 Antibody (G-2) Alexa Fluor® 488 | sc-377170 AF488 | 200 µg/ml | $357.00 | |||
RGL1 Antibody (G-2) Alexa Fluor® 546 | sc-377170 AF546 | 200 µg/ml | $357.00 | |||
RGL1 Antibody (G-2) Alexa Fluor® 594 | sc-377170 AF594 | 200 µg/ml | $357.00 | |||
RGL1 Antibody (G-2) Alexa Fluor® 647 | sc-377170 AF647 | 200 µg/ml | $357.00 | |||
RGL1 Antibody (G-2) Alexa Fluor® 680 | sc-377170 AF680 | 200 µg/ml | $357.00 | |||
RGL1 Antibody (G-2) Alexa Fluor® 790 | sc-377170 AF790 | 200 µg/ml | $357.00 |