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Nbs1 Antibody [DyLight 650]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 650

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View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-06609AF350
Alexa Fluor 405
NBP1-06609AF405
Alexa Fluor 488
NBP1-06609AF488
Alexa Fluor 532
NBP1-06609AF532
Alexa Fluor 594
NBP1-06609AF594
Alexa Fluor 647
NBP1-06609AF647
Alexa Fluor 700
NBP1-06609AF700
Alexa Fluor 750
NBP1-06609AF750
Allophycocyanin
NBP1-06609APC
Biotin
NBP1-06609B
CoraFluor 1
NBP1-06609CL1
DyLight 350
NBP1-06609UV
DyLight 405
NBP1-06609V
DyLight 488
NBP1-06609G
DyLight 550
NBP1-06609R
DyLight 594
NBP1-06609DL594
DyLight 680
NBP1-06609FR
DyLight 755
NBP1-06609IR
FITC
NBP1-06609F
HRP
NBP1-06609H
Janelia Fluor 525
NBP1-06609JF525
Janelia Fluor 549
NBP1-06609JF549
Janelia Fluor 646
NBP1-06609JF646
Janelia Fluor 669
NBP1-06609JF669
PE
NBP1-06609PE
PerCP
NBP1-06609PCP
Unconjugated
NBP1-06609
mFluor Violet 450 SE
NBP1-06609MFV450
mFluor Violet 500 SE
NBP1-06609MFV500
mFluor Violet 610 SE
NBP1-06609MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-06609
BSA Free
NBP1-06609
BSA Free
NBP1-06609SS
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Datasheet
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Support & Research

Nbs1 Antibody [DyLight 650] Summary

Immunogen
Nbs1 Antibody was made to a synthetic peptide made to an internal portion of the mouse NBS1 protein (within residues 350-400). [Swiss-Prot# Q9R207]
Localization
Nucleus. Telomere. Note= Localizes to discrete nuclear foci after treatment with genotoxic agents.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NBN
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 21349997 and 30176843). Mouse reactivity reported in scientific literature.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Nbs1 Antibody [DyLight 650]

  • ATV
  • AT-V1
  • AT-V2
  • Cell cycle regulatory protein p95
  • FLJ10155
  • MGC87362
  • NBN
  • NBS
  • Nbs1
  • NBS1P95
  • Nibrin
  • Nijmegen breakage syndrome 1 (nibrin)
  • Nijmegen breakage syndrome protein 1
  • p95 protein of the MRE11/RAD50 complex
  • p95

Background

NBS1 (Nijmegen breakage syndrome protein 1, Nibrin) is the eukaryotic component of MRN complex (Mre11-Rad50-Nbs1) involved in homologous recombination repair for DNA double-strand breaks (DSBs) and DNA damage-induced checkpoint activation. NBS1, a nuclear protein with a theoretical molecular weight of 65-85 kDa, is composed of 2 functional regions: the forkhead associated (FHA) N-terminal domain (24-83 amino acids) and the breast cancer C-terminus (BRCT) domain (105-181 amino acids). Mutations in the NBN gene are associated with Nijmegen breakage syndrome, characterized by microcephaly, growth retardation, immunodeficiency, and an increased susceptibility to prostate cancer, lung cancer, liver cancer and intrahepatic cholangiocarcinoma (IHC) (1).

In DNA double strand break repair, the FHA/BRCT domains bind DNA damage sensor proteins including gamma H2AX (phospho Ser139), phosphorylated MDC1 and CtIP (CtBP-interacting protein). NBS1 then recruits the other members of the MRN complex, Mre11 and Rad50, to the proximity of DNA DSBs. The MRN complex has also been shown to interact with ATM or ATR kinases in the presence of DSBs or replication fork stalling, respectively. Phosphorylation of NBS1 at Ser 278 and Ser343 in response to ionizing radiation (IR) is dependent on ATM and is important for activation of the S phase checkpoint. The activation of ATM in response to DNA damage is also facilitated by MRN and may lead to induction of apoptosis (2, 3).

References

1. Bian L, Meng Y, Zhang M, Li D. (2019) MRE11-RAD50-NBS1 complex alterations and DNA damage response: implications for cancer treatment. Mol Cancer. 26;18(1):169. PMID: 31767017

2. Zhang Y, Zhou J, Lim CU. (2006) The role of NBS1 in DNA double strand break repair, telomere stability, and cell cycle checkpoint control. Cell Res. 16(1):45-54. PMID: 16467875

3. Komatsu K. NBS1 and multiple regulations of DNA damage response. (2016) J Radiat Res. 57 Suppl 1:i11-i17. PMID: 27068998

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol NBN