UbcH6/UBE2E1 Antibody [HRP] Summary
| Immunogen |
A synthetic peptide made to an N-terminal portion of the human UBCH6 protein (between residues 1-50) [UniProt P51965] |
| Localization |
Nucleus. |
| Isotype |
IgG |
| Clonality |
Polyclonal |
| Host |
Rabbit |
| Gene |
UBE2E1 |
| Purity |
Immunogen affinity purified |
| Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
| Dilutions |
- Immunocytochemistry/ Immunofluorescence
- Immunohistochemistry
- Immunohistochemistry-Paraffin
|
| Application Notes |
Optimal dilution of this antibody should be experimentally determined. |
| Readout System |
|
Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark. |
| Buffer |
PBS |
| Preservative |
No Preservative |
| Purity |
Immunogen affinity purified |
Alternate Names for UbcH6/UBE2E1 Antibody [HRP]
Background
Ubiquitination is a cellular process by which the cell identifies abnormal or short-lived proteins for degradation. Three classes of enzymes are involved in this process, ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBCH6 (UBE2E1 or ubiquitin-conjugating enzyme E2E 1) belongs to the E2 class of enzymes. ISG15, an ubiquitin like protein has been shown to covalently modify UBCH6 as well as forming a thoiester intermediate with ubiquitin. ISG15-modified UBCH6 formed a ubiquitin thioester while the unmodified UBCH6 did not which suggests that ISG156 conjugation suppresses UBCH6 E2 enzyme activity. (PMID: 16428300)
In Spinalcerebellar Ataxia-1 (SCA1), research has shown that UBCH6 ubiquinates Ataxin-1. The overexpression of UBCH6 also reduced the transcriptional repression activity of ataxin-1. It addition it was found that overexpression of UbcH6 reduced the formation of Ataxin-1 aggregated. By modulating the degradation of ataxin-1, UbcH6 can regulate the transcriptional repression activity of ataxin-1 (PMID: 18519031).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Publications for UbcH6/UBE2E1 Antibody (NBP1-78983H) (0)
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Product General Protocols
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Video Protocols
FAQs for UbcH6/UBE2E1 Antibody (NBP1-78983H) (0)
Secondary Antibodies
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Isotype Controls
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