p19ARF/CDKN2A Antibody (5-C3-1) [Biotin] Summary
| Immunogen |
A synthetic peptide containing amino acids 54-75 of the murine p19ARF/CDKN2A. [UniProt# Q64364] |
| Epitope |
The epitope recognized is between amino acids 62-75 of murine p19ARF/CDKN2A. |
| Isotype |
IgG2b |
| Clonality |
Monoclonal |
| Host |
Rat |
| Gene |
Cdkn2a |
| Purity |
Protein A purified |
| Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
| Dilutions |
- CyTOF-ready
- Flow (Intracellular)
- Flow Cytometry
- Immunocytochemistry/ Immunofluorescence
- Immunohistochemistry
- Immunohistochemistry-Frozen
- Immunohistochemistry-Paraffin
- Immunoprecipitation
- Knockout Validated
- Western Blot
|
| Application Notes |
Optimal dilution of this antibody should be experimentally determined. |
| Readout System |
|
Reactivity Notes
Human reactivity reported in scientific literature (PMID: 27177758). The antibody does not react with Golden Syrian hamster. Armenian hamster has not been tested.
Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark. |
| Buffer |
PBS |
| Preservative |
0.05% Sodium Azide |
| Purity |
Protein A purified |
Alternate Names for p19ARF/CDKN2A Antibody (5-C3-1) [Biotin]
Background
In humans, the multiple tumor suppressor 1 locus (MTS1) encodes two unrelated genes, p16INK4a and p19ARF. Although both act as cell proliferation inhibitors, their mechanisms of action are different. p19ARF works as part of a p53-dependent pathway to counter uncontrolled proliferation and oncogenic signals.
Mice lacking the p19ARF gene rapidly develop a broad spectrum of tumors. This result indicates that p19ARF is an important tumor suppressor. In addition, p19ARF helps prevent transforming signals from various onocoproteins via the p53 regulatory loop. These studies and others display the importance of p19ARF in cell cycle control and tumor suppression.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Product General Protocols
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FAQs for p19ARF/CDKN2A Antibody (NB200-174B) (0)
Secondary Antibodies
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Isotype Controls
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