RDH5 Antibody (G-5): sc-377057
- RDH5 Antibody (G-5) is a mouse monoclonal IgG1 κ RDH5 antibody provided at 200 µg/ml
- raised against amino acids 111-150 mapping within an internal region of RDH5 of human origin
- RDH5 Antibody (G-5) is recommended for detection of RDH5 of human origin by WB, IP, IF and ELISA
- Anti-RDH5 Antibody (G-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of RDH5 (G-5): sc-377057.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for RDH5 Antibody (G-5). This work is in progress.
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RDH5 Antibody (G-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects RDH5 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RDH5 (G-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Retinol dehydrogenase 5 (RDH5), also known as 11-cis retinol dehydrogenase or RDH1, is a 318 amino acid protein that belongs to the short-chain dehydrogenases/reductases (SDR) family. RDH5 is primarily located in the retinal pigment epithelium, where RDH5 plays a crucial role in the visual cycle by catalyzing the conversion of all-trans retinol (vitamin A) to 11-cis retinal, the essential chromophore for visual pigments. RDH5 function is vital for the regeneration of photopigments in both cone and rod cells, which is necessary for normal vision. Mutations in the RDH5 gene can lead to fundus albipunctatus, a rare form of stationary night blindness characterized by a delay in the regeneration of these photopigments, underscoring RDH5′s importance in visual health.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RDH5 Antibody (G-5) References:
- Mutations in the gene encoding 11-cis retinol dehydrogenase cause delayed dark adaptation and fundus albipunctatus. | Yamamoto, H., et al. 1999. Nat Genet. 22: 188-91. PMID: 10369264
- Aberrant metabolites in mouse models of congenital blinding diseases: formation and storage of retinyl esters. | Maeda, A., et al. 2006. Biochemistry. 45: 4210-9. PMID: 16566595
- Compound heterozygous RDH5 mutations in familial fleck retina with night blindness. | Hayashi, T., et al. 2006. Acta Ophthalmol Scand. 84: 254-8. PMID: 16637847
- Dual roles for adenomatous polyposis coli in regulating retinoic acid biosynthesis and Wnt during ocular development. | Nadauld, LD., et al. 2006. Proc Natl Acad Sci U S A. 103: 13409-14. PMID: 16938888
- Improvement in rod and cone function in mouse model of Fundus albipunctatus after pharmacologic treatment with 9-cis-retinal. | Maeda, A., et al. 2006. Invest Ophthalmol Vis Sci. 47: 4540-6. PMID: 17003450
- Homozygous deletion related to Alu repeats in RLBP1 causes retinitis punctata albescens. | Humbert, G., et al. 2006. Invest Ophthalmol Vis Sci. 47: 4719-24. PMID: 17065479
- Targeted disruption of the murine retinal dehydrogenase gene Rdh12 does not limit visual cycle function. | Kurth, I., et al. 2007. Mol Cell Biol. 27: 1370-9. PMID: 17130236
- Fundus albipunctatus in a 6-year old girl due to compound heterozygous mutations in the RDH5 gene. | Iannaccone, A., et al. 2007. Doc Ophthalmol. 115: 111-6. PMID: 17476461
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RDH5 Antibody (G-5) | sc-377057 | 200 µg/ml | $316.00 | |||
RDH5 Antibody (G-5) AC | sc-377057 AC | 500 µg/ml, 25% agarose | $416.00 | |||
RDH5 Antibody (G-5) HRP | sc-377057 HRP | 200 µg/ml | $316.00 | |||
RDH5 Antibody (G-5) FITC | sc-377057 FITC | 200 µg/ml | $330.00 | |||
RDH5 Antibody (G-5) PE | sc-377057 PE | 200 µg/ml | $343.00 | |||
RDH5 Antibody (G-5) Alexa Fluor® 488 | sc-377057 AF488 | 200 µg/ml | $357.00 | |||
RDH5 Antibody (G-5) Alexa Fluor® 546 | sc-377057 AF546 | 200 µg/ml | $357.00 | |||
RDH5 Antibody (G-5) Alexa Fluor® 594 | sc-377057 AF594 | 200 µg/ml | $357.00 | |||
RDH5 Antibody (G-5) Alexa Fluor® 647 | sc-377057 AF647 | 200 µg/ml | $357.00 | |||
RDH5 Antibody (G-5) Alexa Fluor® 680 | sc-377057 AF680 | 200 µg/ml | $357.00 | |||
RDH5 Antibody (G-5) Alexa Fluor® 790 | sc-377057 AF790 | 200 µg/ml | $357.00 |