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PIGA Antibody

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Western Blot: PIGA Antibody [NBP2-19827] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE gel, diluted at 1:1000.
Western Blot: PIGA Antibody [NBP2-19827] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE gel, diluted at 1:1000.

Product Details

Summary
Reactivity Mu, Hu, RMSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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PIGA Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human PIGA. The exact sequence is proprietary.
Localization
Endoplasmic reticulum membrane; Single-pass membrane protein
Predicted Species
Human (100%), Rhesus Macaque (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PIGA
Purity
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat (83%), Chicken (80%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol,1% BSA
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for PIGA Antibody

  • class A GlcNAc-inositol phospholipid assembly protein
  • EC 2.4.1.198
  • GlcNAc-PI synthesis protein
  • GPI anchor biosynthesis
  • GPI3
  • phosphatidylinositol glycan anchor biosynthesis, class A
  • phosphatidylinositol glycan, class A (paroxysmal nocturnal hemoglobinuria)
  • phosphatidylinositol N-acetylglucosaminyltransferase subunit A
  • Phosphatidylinositol-glycan biosynthesis class A protein
  • phosphatidylinositol-glycan biosynthesis, class A protein
  • PIG-A

Background

This gene encodes a protein required for synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the biosynthetic pathway of GPI anchor. The GPI anchor is a glycolipid found on many blood cells and which serves to anchor proteins to the cell surface. Paroxysmal nocturnal hemoglobinuria, an acquired hematologic disorder, has been shown to result from mutations in this gene. Alternate splice variants have been characterized. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Bioinformatics

Gene Symbol PIGA
Uniprot