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Orthogonal Strategies: Immunohistochemistry-Paraffin: Collagen VII Antibody [NBP2-37900] - Analysis in human skin and liver tissues using antibody. Corresponding COL7A1 RNA-seq data are presented for the same ...read more
Orthogonal Strategies: Immunohistochemistry-Paraffin: Collagen VII Antibody [NBP2-37900] - Analysis in human skin and liver tissues using antibody. Corresponding COL7A1 RNA-seq data are presented for the same ...read more
Immunohistochemistry-Paraffin: Collagen VII Antibody [NBP2-37900] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Collagen VII Antibody [NBP2-37900] - Staining of human skin shows moderate membranous positivity in epidermal cells.
Immunohistochemistry-Paraffin: Collagen VII Antibody [NBP2-37900] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: Collagen VII Antibody [NBP2-37900] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
This antibody was developed against a recombinant protein corresponding to amino acids: LTLYTLLEGHEVATPATVVPTGPELPVSPVTDLQATELPGQRVRVSWSPVPGATQYRIIVRSTQGVERTLVLPGSQTAFDLDDVQAGLSYTVRVSARVGPREGSASVLTVRREPETPLAVPGLRVVVSDATRVRVAWGPAPGASGFRI
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
COL7A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%)
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified
Alternate Names for Collagen VII Antibody - BSA Free
Collagen 7
collagen alpha-1(VII) chain
collagen VII, alpha-1 polypeptide
collagen, type VII, alpha 1
Collagen-7
EBD1
EBDCT
EBR1
epidermolysis bullosa, dystrophic, dominant and recessive
LC collagen
long chain collagen
Long-chain collagen
Background
Type VII collagen fibrils are composed of three identical alpha collagen chains. It's location is restricted to the basement zone beneath stratified squamous epithelia. It functions as an anchoring fibril between the external epithelia and the underlying stroma. Mutations in the collagen V11 gene are associated with all forms of dystrophic epidermolysis bullosa.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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![Immunohistochemistry-Paraffin: Collagen VII Antibody [NBP2-37900] - Analysis in human skin and liver tissues using antibody. Corresponding COL7A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Collagen-VII-Antibody-Immunohistochemistry-Paraffin-NBP2-37900-img0006.jpg "Immunohistochemistry-Paraffin: Collagen VII Antibody [NBP2-37900] - Analysis in human skin and liver tissues using antibody. Corresponding COL7A1 RNA-seq data are presented for the same tissues.")
![Immunohistochemistry-Paraffin: Collagen VII Antibody [NBP2-37900] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Collagen-VII-Antibody-Immunohistochemistry-Paraffin-NBP2-37900-img0008.jpg "Immunohistochemistry-Paraffin: Collagen VII Antibody [NBP2-37900] - Staining of human liver shows no positivity in hepatocytes as expected.")
![Immunohistochemistry-Paraffin: Collagen VII Antibody [NBP2-37900] - Staining of human skin shows moderate membranous positivity in epidermal cells.](http://images.novusbio.com/fullsize/Collagen-VII-Antibody-Immunohistochemistry-Paraffin-NBP2-37900-img0003.jpg "Immunohistochemistry-Paraffin: Collagen VII Antibody [NBP2-37900] - Staining of human skin shows moderate membranous positivity in epidermal cells.")
![Immunohistochemistry-Paraffin: Collagen VII Antibody [NBP2-37900] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Collagen-VII-Antibody-Immunohistochemistry-Paraffin-NBP2-37900-img0005.jpg "Immunohistochemistry-Paraffin: Collagen VII Antibody [NBP2-37900] - Staining of human skeletal muscle shows no positivity in myocytes as expected.")
![Immunohistochemistry-Paraffin: Collagen VII Antibody [NBP2-37900] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Collagen-VII-Antibody-Immunohistochemistry-Paraffin-NBP2-37900-img0007.jpg "Immunohistochemistry-Paraffin: Collagen VII Antibody [NBP2-37900] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")