CTH Antibody (F-1): sc-374249

CTH Antibody (F-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects CTH protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). CTH Antibody (F-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CTH, or cystathionine γ-lyase, is a crucial enzyme in the trans-sulfuration pathway, primarily located in the cytoplasm, where CTH (F-1) catalyzes the β-disulfide elimination reaction that produces ammonium, pyruvate, and thiocysteine in a pyridoxal phosphate-dependent manner. The thiocysteine generated can subsequently react with other thiols, leading to the production of hydrogen sulfide (H2S), a signaling molecule that plays a vital role in various physiological processes. CTH expression in tissues such as the kidney, liver, vascular smooth muscle cells, and enterocytes underscores CTH′s importance in regulating cellular functions, including cell growth, modulation of neuronal excitability, and relaxation of smooth muscle cells. Furthermore, the endogenous production of H2S is essential for maintaining vascular homeostasis and influencing blood pressure regulation. Mutations in the CTH gene can lead to cystathioninuria, an autosomal recessive disorder characterized by the accumulation of cystathionine in plasma and increased urinary excretion, highlighting the clinical significance of this enzyme in metabolic pathways.

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CTH Antibody (F-1) References:

1. Cloning and nucleotide sequence of human liver cDNA encoding for cystathionine gamma-lyase.  |  Lu, Y., et al. 1992. Biochem Biophys Res Commun. 189: 749-58. PMID: 1339280
2. Murine cystathionine gamma-lyase: complete cDNA and genomic sequences, promoter activity, tissue distribution and developmental expression.  |  Ishii, I., et al. 2004. Biochem J. 381: 113-23. PMID: 15038791
3. Single nucleotide polymorphism in CTH associated with variation in plasma homocysteine concentration.  |  Wang, J., et al. 2004. Clin Genet. 65: 483-6. PMID: 15151507
4. Endogenous production of hydrogen sulfide in mammals.  |  Kamoun, P. 2004. Amino Acids. 26: 243-54. PMID: 15221504
5. [H2S, a new neuromodulator].  |  Kamoun, P. 2004. Med Sci (Paris). 20: 697-700. PMID: 15329822
6. Cystathionine gamma-lyase overexpression inhibits cell proliferation via a H2S-dependent modulation of ERK1/2 phosphorylation and p21Cip/WAK-1.  |  Yang, G., et al. 2004. J Biol Chem. 279: 49199-205. PMID: 15347670
7. New roles for cysteine and transsulfuration enzymes: production of H2S, a neuromodulator and smooth muscle relaxant.  |  Dominy, JE. and Stipanuk, MH. 2004. Nutr Rev. 62: 348-53. PMID: 15497768
8. Pro-apoptotic effect of endogenous H2S on human aorta smooth muscle cells.  |  Yang, G., et al. 2006. FASEB J. 20: 553-5. PMID: 16507767
9. Hydrogen sulfide is a novel prosecretory neuromodulator in the Guinea-pig and human colon.  |  Schicho, R., et al. 2006. Gastroenterology. 131: 1542-52. PMID: 17101327
10. Contractile and vasorelaxant effects of hydrogen sulfide and its biosynthesis in the human internal mammary artery.  |  Webb, GD., et al. 2008. J Pharmacol Exp Ther. 324: 876-82. PMID: 18029544
11. Association between an indel polymorphism within CTH and the risk of sudden cardiac death in a Chinese population.  |  Zhou, W., et al. 2020. Leg Med (Tokyo). 46: 101736. PMID: 32563979
12. Mutations in the TP53, VEGFA, and CTH Genes as Key Molecular Markers for the Diagnosis of Glioblastoma.  |  Khalil, SS. and Salihi, A. 2024. Cureus. 16: e61165. PMID: 38933650

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CTH Antibody (F-1) has 27 citations in a variety of scientific publications.

Primary antibodies like Anti-CTH Antibody (F-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CTH Antibody (F-1) Specifications

Alternate Product Name: Cystathionase

Product Clone Name: F-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 43-75

Epitope Target: CTH

Epitope Species: human

Product Reactivity/Detection: CTH isoforms 1 and 2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 27 citations in various scientific publications

Available Conjugates/Forms for CTH Antibody (F-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin