Calpain 2 Antibody (F-11): sc-373967

Calpain 2 Antibody (F-11) is a mouse monoclonal IgG2b kappa light chain antibody that detects Calpain 2 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-Calpain 2 antibody (F-11) is available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. Calpain 2, a crucial intracellular calcium-dependent cysteine protease, plays a significant role in regulating cellular processes by cleaving cytoskeletal and submembranous proteins, which is vital for cytoskeletal reorganization and muscle protein degradation. Calpain 2 is primarily located in the cytoplasm and activates in response to elevated intracellular calcium levels, highlighting its importance in cellular signaling pathways and muscle function. Additionally, Calpain 2 exists as a heterodimer, consisting of a small regulatory subunit and one of three large catalytic subunits, which include Calpain 1, Calpain 2, and Calpain p94. Calpain 2 activity is tightly regulated by calpastatin, which inhibits both proteolytic activity and membrane binding, with calpastatin existing in tissue and erythrocyte types due to alternative splicing and proteolytic processing. This regulation is essential for maintaining cellular homeostasis and preventing excessive proteolysis, underscoring Calpain 2′s importance in various physiological and pathological processes.

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Calpain 2 Antibody (F-11) References:

1. Calpain-2 Inhibitor Therapy Reduces Murine Colitis and Colitis-associated Cancer.  |  Rose, AH., et al. 2015. Inflamm Bowel Dis. 21: 2005-15. PMID: 26076056
2. Calpain-2 activity promotes aberrant endoplasmic reticulum stress-related apoptosis in hepatocytes.  |  Xie, RJ., et al. 2020. World J Gastroenterol. 26: 1450-1462. PMID: 32308346
3. Calpain-2 as a therapeutic target in repeated concussion-induced neuropathy and behavioral impairment.  |  Wang, Y., et al. 2020. Sci Adv. 6: PMID: 32937436
4. Calpain-2 activation in mouse hippocampus plays a critical role in seizure-induced neuropathology.  |  Wang, Y., et al. 2021. Neurobiol Dis. 147: 105149. PMID: 33132163
5. Calpain-1 and Calpain-2 in the Brain: New Evidence for a Critical Role of Calpain-2 in Neuronal Death.  |  Wang, Y., et al. 2020. Cells. 9: PMID: 33339205
6. Calpain-2 regulates hypoxia/HIF-induced plasticity toward amoeboid cancer cell migration and metastasis.  |  Te Boekhorst, V., et al. 2022. Curr Biol. 32: 412-427.e8. PMID: 34883047
7. Sustained over-expression of calpain-2 induces age-dependent dilated cardiomyopathy in mice through aberrant autophagy.  |  Ji, XY., et al. 2022. Acta Pharmacol Sin. 43: 2873-2884. PMID: 35986214
8. Changes in calpain-2 expression during glioblastoma progression predisposes tumor cells to temozolomide resistance by minimizing DNA damage and p53-dependent apoptosis.  |  Stillger, MN., et al. 2023. Cancer Cell Int. 23: 49. PMID: 36932402
9. Calpain-2 protein influences chikungunya virus replication and regulates vimentin rearrangement caused by chikungunya virus infection.  |  Li, J., et al. 2023. Front Microbiol. 14: 1229576. PMID: 37928675
10. LncRNA FAISL Inhibits Calpain 2-Mediated Proteolysis of FAK to Promote Progression and Metastasis of Triple Negative Breast Cancer.  |  Zhang, Y., et al. 2024. Adv Sci (Weinh). 11: e2407493. PMID: 39287113

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Calpain 2 Antibody (F-11) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-Calpain 2 Antibody (F-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Calpain 2 Antibody (F-11) Specifications

Alternate Product Name: Calpain (calcium-dependent cysteine protease) 2

Product Clone Name: F-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 2-27

Epitope Target: Calpain 2

Epitope Species: human

Product Reactivity/Detection: Calpain 2 precursor

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for Calpain 2 Antibody (F-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Calpain 2 antibody is Calpain 2 Antibody (E-10): sc-373966.