Aldehyde dehydrogenase 2/ALDH2 Antibody (3D12): sc-100496

ALDH2 Antibody (3D12) is a mouse monoclonal IgG2a kappa light chain antibody that detects aldehyde dehydrogenase 2 (ALDH2) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). ALDH2 (3D12) antibody is available in a non-conjugated form, allowing for versatile experimental use. ALDH2 plays a crucial role in the detoxification of aldehydes, particularly in the metabolism of alcohol-derived acetaldehyde, which is vital for preventing toxic accumulation in the body. This enzyme is predominantly located in the mitochondria, where ALDH2 catalyzes the conversion of acetaldehyde to acetate, a less harmful substance that can be further metabolized for energy. The mitochondrial localization of ALDH2 allows for efficient detoxification within the cellular powerhouse, thereby protecting cells from oxidative stress and damage. Notably, variations in the ALDH2 gene can lead to a deficiency in enzyme activity, which is particularly prevalent in certain populations, such as 50% of Asians, resulting in alcohol intolerance and a reduced risk of alcoholism-related liver disease. The interaction of ALDH2 with other proteins, such as ALDH1A1, further underscores ALDH2′s importance in metabolic pathways, making anti-ALDH2 antibody (3D12) an essential tool for researchers studying alcohol metabolism and related disorders.

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Aldehyde dehydrogenase 2/ALDH2 Antibody (3D12) References:

1. Molecular analysis of two closely related mouse aldehyde dehydrogenase genes: identification of a role for Aldh1, but not Aldh-pb, in the biosynthesis of retinoic acid.  |  Hsu, LC., et al. 1999. Biochem J. 339 (Pt 2): 387-95. PMID: 10191271
2. Eukaryotic aldehyde dehydrogenase (ALDH) genes: human polymorphisms, and recommended nomenclature based on divergent evolution and chromosomal mapping.  |  Vasiliou, V., et al. 1999. Pharmacogenetics. 9: 421-34. PMID: 10780262
3. Aldehyde dehydrogenase (ALDH) 2 associates with oxidation of methoxyacetaldehyde; in vitro analysis with liver subcellular fraction derived from human and Aldh2 gene targeting mouse.  |  Kitagawa, K., et al. 2000. FEBS Lett. 476: 306-11. PMID: 10913633
4. cDNA cloning and expression of a human aldehyde dehydrogenase (ALDH) active with 9-cis-retinal and identification of a rat ortholog, ALDH12.  |  Lin, M. and Napoli, JL. 2000. J Biol Chem. 275: 40106-12. PMID: 11007799
5. Negative regulation of the murine cytosolic aldehyde dehydrogenase-3 (Aldh-3c) gene by functional CYP1A1 and CYP1A2 proteins.  |  Vasiliou, V., et al. 1992. Biochem Biophys Res Commun. 187: 413-9. PMID: 1520328
6. Analysis and update of the human aldehyde dehydrogenase (ALDH) gene family.  |  Vasiliou, V. and Nebert, DW. 2005. Hum Genomics. 2: 138-43. PMID: 16004729
7. Tissue- and species-specific expression patterns of class I, III, and IV Adh and Aldh 1 mRNAs in rodent embryos.  |  Westerlund, M., et al. 2005. Cell Tissue Res. 322: 227-36. PMID: 16047160
8. Activity of alcohol dehydrogenase (ADH) isoenzymes and aldehyde dehydrogenase (ALDH) in the sera of patients with breast cancer.  |  Jelski, W., et al. 2006. J Clin Lab Anal. 20: 105-8. PMID: 16721836
9. The role of ALDH2 in tumorigenesis and tumor progression: Targeting ALDH2 as a potential cancer treatment.  |  Zhang, H. and Fu, L. 2021. Acta Pharm Sin B. 11: 1400-1411. PMID: 34221859
10. p53 protects against alcoholic fatty liver disease via ALDH2 inhibition.  |  Yao, P., et al. 2023. EMBO J. 42: e112304. PMID: 36825429
11. Myocardial reperfusion injury exacerbation due to ALDH2 deficiency is mediated by neutrophil extracellular traps and prevented by leukotriene C4 inhibition.  |  Yang, K., et al. 2024. Eur Heart J. 45: 1662-1680. PMID: 38666340
12. Isolation and characterization of aldehyde dehydrogenase isozymes from usual and atypical human livers.  |  Ikawa, M., et al. 1983. J Biol Chem. 258: 6282-7. PMID: 6189823

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Aldehyde dehydrogenase 2/ALDH2 Antibody (3D12) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-Aldehyde dehydrogenase 2/ALDH2 Antibody (3D12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Aldehyde dehydrogenase 2/ALDH2 Antibody (3D12) Specifications

Alternate Product Name: ALDH2

Product Clone Name: 3D12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 408-518

Epitope Target: ALDH2

Epitope Species: human

Product Reactivity/Detection: ALDH2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for Aldehyde dehydrogenase 2/ALDH2 Antibody (3D12): N/A

The preferred Aldehyde dehydrogenase /2/ALDH1A1/2/3/ALDH2 antibody is Aldehyde dehydrogenase /2/ALDH1A1/2/3/ALDH2 Antibody (H-8): sc-166362.